Near Infrared Imaging of EGFR of Oral Squamous Cell Carcinoma in Mice Administered Arsenic Trioxide 英文参考文献.docVIP

下载本文档

5
0
约3.76万字
约 7页
2017-05-12 发布于上海
举报
版权申诉

Near Infrared Imaging of EGFR of Oral Squamous Cell Carcinoma in Mice Administered Arsenic Trioxide 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Near Infrared Imaging of EGFR of Oral Squamous Cell Carcinoma in Mice Administered Arsenic Trioxide 英文参考文献

NearInfraredImagingofEGFRofOralSquamousCell CarcinomainMiceAdministeredArsenicTrioxide LingboZhang1.,KezhengWang2.,FalinZhao3,WeipingHu1,JunjieChen4,GregoryM.Lanza4, BaozhongShen2*,BinZhang1* 1Stomatology Department, Institute of Hard Tissue Development and Regeneration, 2nd Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China, 2RadiologyDepartmentandMolecularImagingCenter,4thAffiliatedHospital,HarbinMedicalUniversity,Harbin,Heilongjiang,China,3SchoolofHealthManagement, HangzhouNormalUniversity,Hangzhou,Zhejiang, China,4DivisionofCardiologyandC-TRAIN,WashingtonUniversitySchoolofMedicine,St.Louis,Missouri,United StatesofAmerica Abstract Background: The effectiveness of near-infrared imaging (NIR) interrogation of epidermal growth factor receptor (EGFR) expression as a sensitive biomarker of oral squamous cell carcinoma (OSCC) response to arsenic trioxide therapy was studiedinmice. MaterialandMethods:A431OSCCinvitrowereexposedto0mM,0.5mM,2.5mM,or5mMofAs2O3for0h,24h,48hand 72h. Confocal microscopy and flow cytometry confirmed EGFR expression and demonstrated a sensitivity dose-related signaldeclinewithAs2O3treatment.Next,micewithpharynx-implantedA431cellsreceivedAs2O3i.p.every48hat0.0,0.5, 2.5,or5mg/kg/day(n=6/group)fromday0to10.AnintravenousNIRprobe,EGF-Cy5.5,wasinjectedatbaselineandon days4,8,and12fordynamicNIRimaging.Tumorvolumeandbodyweightsweremeasuredthreetimesweekly. Results:Invitro,A431EGFRexpressionwaswellappreciatedinthecontrolsanddecreased(p,0.05)withincreasingAs2O3 doseandtreatmentduration.InvivoEGFRNIRtumorsignalintensitydecreased(p,0.05)inAs2O3treatedgroupsversus controlsfromdays4to12,consistentwithincreasingdosage.Tumorvolumediminishedinadose-relatedmannerwhile body weight was unaffected. Immunohistochemical staining of excised tumors confirmed that EGFR expression was reducedbyAs2O3treatmentinadoseresponsivepattern. Conclusion:ThisstudydemonstratesforthefirsttimethatOSCCcanbeinterrogatedinvivobyNIRmolecularimagingof theEGFRand