Oligonucleotide Based Magnetic Bead Capture of Onchocerca volvulus DNA for PCR Pool Screening of Vector Black Flies 英文参考文献.docVIP
- 1、本文档共5页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Oligonucleotide Based Magnetic Bead Capture of Onchocerca volvulus DNA for PCR Pool Screening of Vector Black Flies 英文参考文献
OligonucleotideBasedMagneticBeadCaptureof
OnchocercavolvulusDNAforPCRPoolScreeningof
VectorBlackFlies
HemavathiGopal1,HassanK.Hassan2,MarioA.Rodr?′guez-Pe′rez1,LaurentD.Toe′3,SaraLustigman4,
ThomasR.Unnasch2*
1Centro de Biotecnolog?′a Geno′mica, Instituto Polite′cnico Nacional, Reynosa, Tamaulipas, Me′xico, 2Department of Global Health, Global Health Infectious Disease
ResearchProgram,UniversityofSouthFlorida,Tampa,Florida,UnitedStatesofAmerica,3Multi-DiseaseSurveillanceCentre,Ouagadougou,BurkinaFaso,4Laboratoryof
MolecularParasitology,LindsleyF.KimballResearchInstitute,NewYorkBloodCenter,NewYork,NewYork,UnitedStatesofAmerica
Abstract
Background:EntomologicalsurveysofSimuliumvectorsareanimportantcomponentinthecriteriausedtodetermineif
Onchocercavolvulustransmissionhasbeeninterruptedandiffocaleliminationoftheparasitehasbeenachieved.However,
becauseinfectioninthevectorpopulationisquiterareinareaswherecontrolhassucceeded,largenumbersoffliesneedto
be examined to certify transmission interruption. Currently, this is accomplished through PCR pool screening of large
numbersofflies.Theefficiencyofthisprocessislimitedbythesizeofthepoolsthatmaybescreened,whichisinturn
determined by the constraints imposed by the biochemistry of the assay. The current method of DNA purification from
poolsofvectorblackfliesreliesuponsilicaadsorption.Thismethodcanbeappliedtoscreenpoolscontainingamaximum
of50individuals(fromtheLatinAmericanvectors)or100individuals(fromtheAfricanvectors).
Methodology/PrincipalFindings:WehaveevaluatedanalternativemethodofDNApurificationforpoolscreeningofblack
flies which relies upon oligonucleotide capture of Onchocerca volvulus genomic DNA from homogenates prepared from
poolsofLatinAmericanandAfricanvectors.TheoligonucleotidecaptureassaywasshowntoreliablydetectoneO.volvulus
infectivelarvainpoolscontaining200AfricanorLatinAmericanflies,representingatwo-fourfoldimprovementoverthe
conventional assay. The capture assay requires an equivalent amount of technical time
您可能关注的文档
- Octacalcium phosphate crystals directly stimulate expression of inducible nitric oxide synthase through p38 and JNK mitogen-activated protein kinases in articular chondrocytes 英文参考文献.doc
- OCP3 is an important modulator of NPR1-mediated jasmonic acid-dependent induced defenses in Arabidopsis 英文参考文献.doc
- Ocular Drug Delivery for Glaucoma Management 英文参考文献.doc
- Ocular medicines in children the regulatory situation related to clinical research 英文参考文献.doc
- Ocular lesions in 1,000 consecutive HIV-positive patients in India a long-term study 英文参考文献.doc
- Ocular sonography in patients with raised intracranial pressure the papilloedema revisited 英文参考文献.doc
- Ocular fundus manifestation of two patients following long-term chloroquine therapy a case report 英文参考文献.doc
- Oculomotor Evidence for Top-Down Control following the Initial Saccade 英文参考文献.doc
- Ocular Sequelae of Congenital Toxoplasmosis in Brazil Compared with Europe 英文参考文献.doc
- Oct4-Induced Reprogramming Is Required for Adult Brain Neural Stem Cell Differentiation into Midbrain Dopaminergic Neurons 英文参考文献.doc
文档评论(0)