On-Chip Immunoassay for Determination of Urinary Albumin 英文参考文献.docVIP

Sensors 2009, 9, 10066-10079; doi:10.3390/OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article On-Chip Immunoassay for Determination of Urinary Albumin Wanida Laiwattanapaisal 1,*, Temsiri Songjaroen 2, Thitima Maturos 3, Tanom Lomas 3, Assawapong Sappat 3 and Adisorn Tuantranont 3 1 Centre for Excellence in Omics-Nano Medical Technology Project Development, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand 2 Graduate Programme in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand; E-Mail: stemsiri@ 3 Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Centre, Pathum Thani, 12120, Thailand; E-Mails: thitima.maturos@nectec.or.th (T.M.); tanom.lomas@nectec.or.th (T.L.); assawapong.sappat@nectec.or.th (A.S.); adisorn.tuantranont@ectec.or.th (A.T.) * Author to whom correspondence should be addressed; E-Mail: wanida.k@chula.ac.th; Tel.: +66-022–181-081; Fax: +66-022–181-082. Received: 11 October 2009; in revised form: 13 November 2009 / Accepted: 30 November 2009 / Published: 10 December 2009 Abstract: An immunoassay performed on a portable microfluidic device was evaluated for the determination of urinary albumin. An increase in absorbance at 500 nm resulting from immunoagglutination was monitored directly on the poly(dimethylsiloxane) (PDMS) microchip using a portable miniature fibre-optic spectrometer. A calibration curve was linear up to 10 mg L–1 (r2 = 0.993), with a detection limit of 0.81 mg L–1 (S/N = 3). The proposed system showed good precision, with relative standard deviations (RSDs) of 5.1%, when evaluated with 10 mg L–1 albumin (n = 10). Determination of urinary albumin with the proposed system gave results highly similar to those determined by the conventional spectrophotometric method using immunoturbi