Optimization and Comparison of Five Methods for Extraction of Coniferyl Ferulate from Angelica sinensis 英文参考文献.docVIP

Molecules 2009, 14, 555-565; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Optimization and Comparison of Five Methods for Extraction of Coniferyl Ferulate from Angelica sinensis Jing-Jing Xie 1, Jia Lu 1, Zheng-Ming Qian 1, Yue Yu 1, Jin-Ao Duan 2 and Shao-Ping Li 1,2,* 1 Institute of Chinese Medical Sciences, University of Macau, Macao, P.R. China 2 Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, P.R. China * Author to whom correspondence should be addressed; E-mail: lishaoping@; Tel.: +853-8397 4692; Fax: +853-2884 1358. Received: 15 November 2008; in revised form: 15 January 2009 / Accepted: 19 January 2009 / Published: 23 January 2009 Abstract: Coniferyl ferulate, which is noted for its multiple pharmacological activities and chemical instability, is abundant in Angelica sinensis. In this paper, five methods, namely sonication extraction (SE), pressurized liquid extraction (PLE), supercritical fluid extraction (SFE), hydrodistillation (HD) and decoction (DC) for extraction of coniferyl ferulate, as well as ferulic acid, Z/E-ligustilide and Z/E-butylidenephthalide, from A. sinensis were optimized and compared. The results showed that the order of extraction efficiency was: PLE≈SESFEHD, DC. The compositions of the SE, PLE and SFE extracts, which had a high ratio of coniferyl ferulate, were very similar, while no coniferyl ferulate was obtained by HD and DC, though they had high selectivity for the extraction of ligustilide and ferulic acid, respectively. It was noteworthy that the content of ligustilide and coniferyl ferulate was not detectable in the decoction, the commonly used oral administration form of Traditional Chinese Medicines in clinical practice. Keywords: Hydrodistillation; Supercritical fluid extraction; Pressurized liquid extraction; Angelica sin