Oral Administration of Shark Type II Collagen Suppresses Complete Freund’s Adjuvant-Induced Rheumatoid Arthritis in Rats 英文参考文献.docVIP

Pharmaceuticals 2012, 5, 339-352; doi:10.3390/ph5040339 OPEN ACCESS Pharmaceuticals ISSN 1424-8247 /journal/pharmaceuticals Article Oral Administration of Shark Type II Collagen Suppresses Complete Freund’s Adjuvant-Induced Rheumatoid Arthritis in Rats Lijuan Chen 1, Bin Bao 1, Nanping Wang 2, Jing Xie 1 and Wenhui Wu 1,* 1 Shanghai Ocean University, No. 999, Hu Cheng Loop-road, Lingang New City, Shanghai 201306, China; E-Mail: Clijuan_05shipin@163.com (L.C.) 2 Shanghai Fisheries Research Institute, No. 265 Jiamusi Road, Yangpu District, Shanghai 200433, China; E-Mail: yujiash@ * Author to whom correspondence should be addressed; E-Mail: whwu@; Tel.: +86-21-6190-00388; Fax: +86-21-6190-0354. Received: 7 November 2011; in revised form: 12 February 2012 / Accepted: 21 March 2012 / Published: 28 March 2012 Abstract: Objective: Shark type II collagen (SCII) is extracted as a glycoprotein from the cartilage of blue shark (Prionace glauca). We aim to confirm the effects of oral tolerance of SCII on inflammatory and immune responses to the ankle joint of rheumatoid-arthritis rats induced by Complete Freund’s Adjuvant (CFA). Materials and Methods: The onset of rheumatoid arthritis (RA) was observed 14 ± x days after injection of CFA. Rats in the control group were treated with acetic acid by oral administration (0.05 mmol kg?1d?1, days 14–28), while rats in experimental groups were treated by oral administration with SCII (1 or 3 mg kg?1d?1, days 14–28), Tripterygium wilfordii polyglycosidium (TWP) (10 mg kg?1d?1, days 14–28), and bovine type II collagen from US (US-CII) (1 mg kg?1d?1, days 14–28), respectively. The severity of arthritis was evaluated by the articular swelling. The immunological indexes observed included delayed type hypersensitivity (DTH) reaction, the level of interleukins 10 (IL-10) in rat blood serum and morphological characterization. Mixed lymphocyte culture (MLC)