Oviposition-Stimulant and Ovicidal Activities of Moringa oleifera Lectin on Aedes aegypti 英文参考文献.docVIP

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Oviposition-Stimulant and Ovicidal Activities of Moringa oleifera Lectin on Aedes aegypti 英文参考文献.doc

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Oviposition-Stimulant and Ovicidal Activities of Moringa oleifera Lectin on Aedes aegypti 英文参考文献

Oviposition-StimulantandOvicidalActivitiesofMoringa oleiferaLectinonAedesaegypti NatalyDinizdeLimaSantos1,Ke′ziaSantanadeMoura1,ThiagoHenriqueNapolea?o1,GeanneKarla NovaisSantos2,LuanaCassandraBreitenbachBarrosoCoelho1,DanielaMariadoAmaral FerrazNavarro2,Patr?′ciaMariaGuedesPaiva1* 1DepartamentodeBioqu?′mica,CentrodeCie?nciasBiolo′gicas,UniversidadeFederaldePernambuco,CidadeUniversita′ria,Recife,Pernambuco,Brazil,2Departamentode Qu?′micaFundamental,CentrodeCie?ncias ExatasedaNatureza,UniversidadeFederaldePernambuco,CidadeUniversita′ria, Recife,Pernambuco,Brazil Abstract Background:NaturalinsecticidesagainstthevectormosquitoAedesaegyptihavebeentheobjectofresearchduetotheir highlevelofeco-safety.Thewater-solubleMoringaoleiferalectin(WSMoL)isalarvicidalagentagainstA.aegypti.Thiswork reportstheeffectsofWSMoLonovipositionandegghatchingofA.aegypti. Methodology/Principal Findings: WSMoL crude preparations (seed extract and 0–60 protein fraction), at 0.1mg/mL protein concentration, did not affect oviposition, while A. aegypti gravid females laid their eggs preferentially (73%) in vessels containing isolated WSMoL (0.1mg/mL), compared with vessels containing only distilled water (control). Volatile compounds were not detected in WSMoL preparation. The hatchability of fresh eggs deposited in the solutions in the ovipositionassaywasevaluated.Thenumbersofhatchedlarvaeinseedextract,0–60proteinfractionandWSMoLwere 4568.7%,20611%and5567.5%,respectively,significantly(p,0.05)lowerthanincontrolscontainingonlydistilledwater (75–95%).Embryoswerevisualizedinsidefreshcontroleggs,butnotwithineggsthatwerelaidandmaintainedinWSMoL solution. Ovicidal activity was also assessed using stored A. aegypti eggs. The protein concentrations able to reduce the hatchingrateby50%(EC50)were0.32,0.16and0.1mg/mLforseedextract,0–60proteinfractionandWSMoL,respectively. TheabsenceofhatchingofstoredeggstreatedwithWSMoLat0.3mg/mL(EC99)aftertransfertomediumwithoutlectin indicatesthatembryoswithintheeggs