HPLC method for the determination of two hepatitis B Qingrejiedu film content of flavonoids.docVIP

HPLC method for the determination of two hepatitis B Qingrejiedu film content of flavonoids [Abstract] Objective To establish a high performance liquid chromatography (HPLC method for simultaneous determination of two hepatitis B Qingrejiedu film flavonoids (quercetin, kaempferol content. Methods for the Yi Lite-C18 column column (250mm 4.6mm , 5m, mobile phase of acetonitrile - methanol -0.2% phosphoric acid (10:45:60, detection wavelength 370nm, flow rate 1.0ml/min, injection volume was 20l, the column temperature was 35 . the results of quercetin, kaempferol peptide in the linear range was 4.2 ~ 25.2g/ml (r = 0.9994,0.97 ~ 5.82g/ml (r = 0.9996, average recovery was 97.56%, 98.76%, RSD was 1.62%, 1.52% . Conclusion The method is fast, simple, accurate, reproducible, and can be used for hepatitis B Qingrejiedu the quality control. [Keywords:] Hepatitis B Qingrejiedu chip high pressure liquid chromatography quality control of quercetin kaempferol Hepatitis B virus carriers, Zheng Jian jaundice (with or without jaundice, fever (or low-grade fever, red tongue, thick and greasy tongue coating, slippery pulse string, dry mouth, bitter or sticky smelly, tired of the oil, gastrointestinal discomfort. Hepatitis B heat detoxification chip is cuspidatum, capillaris, Menispermi, diffusa, licorice, Polygonum, etc. made of liver and gallbladder damp-heat for acute and chronic viral hepatitis B early or active treatment. which diffusa important component of the active ingredient flavonoids are heat, detoxification, blood circulation, diuretic effect, can be used to treat various types of cancer and inflammation [1]. Qingrejiedu film hepatitis B Determination of flavonoids rarely reported in the literature, the author High performance liquid chromatography (HPLC method for simultaneous determination of hepatitis B Qingrejiedu film quercetin, kaempferol content for the evaluation of hepatitis B Qingrejiedu Tablets provide a scientific basis. An instrument and reagent