Polysaccharides Coatings on Medical-Grade PVC A Probe into Surface Characteristics and the Extent of Bacterial Adhesion 英文参考文献.docVIP
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Polysaccharides Coatings on Medical-Grade PVC A Probe into Surface Characteristics and the Extent of Bacterial Adhesion 英文参考文献
Molecules 2010, 15, 1007-1027; doi:10.3390/molecule
OPEN ACCESS
molecules
ISSN 1420-3049
/journal/molecules
Article
Polysaccharides Coatings on Medical-Grade PVC: A Probe into
Surface Characteristics and the Extent of Bacterial Adhesion
Ahmad Asadinezhad 1, Igor Novák 2, Marián Lehocky 3,*, Franti?ek Bílek 1, Alenka Vesel 4, Ita
Junkar 4, Petr Sáha 1 and Anton Popelka 2
1
Polymer Centre, Faculty of Technology, Tomas Bata University in Zlín, T.G.M Sq. 275, 762 72
Zlín, Czech Republic
2
Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 842 36 Bratislava, Slovakia
3
Tomas Bata University in Zlín, T.G.M. Sq. 5555, 760 01 Zlín, Czech Republic
4
Plasma Laboratory, Department of Surface Engineering, Jo?ef Stefan Institute, Jamova cesta 39, SI-
1000 Ljubljana, Slovenia
* Author to whom correspondence should be addressed: Email: lehocky@post.cz;
Tel.: +420 608616048; Fax: +420 576031444.
Received: 4 January 2010; in revised form: 22 February 2010 / Accepted: 23 February 2010 /
Published: 23 February 2010
Abstract: Medical-grade polyvinyl chloride was coated by polysaccharides through a
novel physicochemical approach. An initial surface activation was performed foremost via
diffuse coplanar surface barrier discharge plasma in air at ambient temperature and
pressure. Then, radical graft copolymerization of acrylic acid through grafting-from
pathway was directed to render a well-defined brush of high density, and finally a chitosan
monolayer and chitosan/pectin alternating multilayer were bound onto the functionalized
surfaces. Surface characteristics were systematically investigated using several probe
techniques. In vitro bacterial adhesion and biofilm formation assays indicated that a single
chitosan layer was incapable of hindering the adhesion of a Staphylococcus aureus
bacterial strain, while up to 30% reduction was achieved by the chitosan/pectin layered
asse
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