Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography 英文参考文献.docVIP

Molecules 2012, 17, 9002-9009; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography Guilong Yan 1,2,3, Lilian Ji 2,3, Yuming Luo 2 and Yonghong Hu 1,* 1 College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China 2 Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China 3 Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian 223300, China * Author to whom correspondence should be addressed; E-Mail: hyh@; Tel.: +86-25-8358-7108. Received: 20 June 2012; in revised form: 18 July 2012 / Accepted: 21 July 2012 / Published: 27 July 2012 Abstract: A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. The two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water (1:4:2:3, v/v/v/v) was selected. From 540 mg of the n-butanol fraction of Eupatorium lindleyanum DC., 10.8 mg of 3β-hydroxy-8β-[4-hydroxy- tigloyloxy]-costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and 1H-NMR. Keywords: Eupatorium lindleyanum DC.; high-speed counter-current chromatography; sesquiterpenoid lactone; separation  Molecules 2012, 17 9003 1. Introduction Eupatorium lindleyanum DC. (family Compositae) is a traditional Chinese herb. Its aerial part, called ‘Yemazhui’, shows antihistaminic, antibacterial and ant