Prevalence and Factors Associated with Leishmania infantum Infection of Dogs from an Urban Area of Brazil as Identified by Molecular Methods 英文参考文献.docVIP
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Prevalence and Factors Associated with Leishmania infantum Infection of Dogs from an Urban Area of Brazil as Identified by Molecular Methods 英文参考文献
PrevalenceandFactorsAssociatedwithLeishmania
infantumInfectionofDogsfromanUrbanAreaofBrazil
asIdentifiedbyMolecularMethods
WendelCoura-Vital1,2,MarcosJose′ Marques3,VanjaMariaVeloso4,BrunoMendesRoatt2,RodrigoDian
deOliveiraAguiar-Soares2,LeviEduardoSoaresReis2,SamuelLeo?ncioBraga2,MariaHelenaFranco
Morais1,5,AlexandreBarbosaReis2,Maria?ngelaCarneiro1,6*
1DepartamentodeParasitologia,InstitutodeCie?nciasBiolo′gicas,UniversidadeFederaldeMinasGerais,BeloHorizonte,MinasGerais,Brazil,2Nu′cleodePesquisasem
Cie?nciasBiolo′gicas,InstitutodeCie?nciasExataseBiolo′gicas,UniversidadeFederaldeOuroPreto,OuroPreto,MinasGerais,Brazil,3DepartamentodeCie?nciasBiolo′gicas,
UniversidadeFederaldeAlfenas,Alfenas,MinasGerais,Brazil,4DepartamentodeFarma′cia, EscoladeFarma′cia, UniversidadeFederaldeOuroPreto,OuroPreto,Minas
Gerais, Brazil, 5Gere?ncia Regional de Controle de Zoonoses, Secretaria Municipal de Sau′de, Prefeitura de Belo Horizonte, Belo Horizonte, Minas Gerais, Brazil, 6Po′s-
Graduac?a?o emInfectologiaeMedicinaTropical,FaculdadedeMedicina,UniversidadeFederaldeMinasGerais,BeloHorizonte,MinasGerais,Brazil
Abstract
Background: Variousfactors contribute totheurbanizationof thevisceralleishmaniasis (VL), including thedifficulties of
implementingcontrolmeasuresrelatingtothedomesticreservoir.Theaimofthisstudywastodeterminetheprevalenceof
canine visceral leishmaniasis in an urban endemic area in Brazil and the factors associated with Leishmania infantum
infectionamongseronegativeandPCR-positivedogs.
Methodology:Across-sectionalstudywasconductedinBeloHorizonte,MinasGerais,Brazil.Bloodsampleswerecollected
from1,443dogs.Serologywascarriedoutbyusingtwoenzyme-linkedimmunosorbentassays(Biomanguinhos/FIOCRUZ/
RJand‘‘inhouse’’),andmolecularmethodsweredeveloped,includingPCR-RFLP.Toidentifythefactorsassociatedwith
earlystagesofinfection,onlyseronegative(n=1,213)animalswereevaluated.Theseanimalsweredividedintotwogroups:
PCR-positive(n=296)andPCR-negative(n=917)forL.infantumDNA.Acomparis
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