Prevalence of aac(6)-Ib-cr plasmid-mediated and chromosome-encoded fluoroquinolone resistance in Enterobacteriaceae in Italy 英文参考文献.docVIP

下载本文档

15
0
约1.71万字
约 5页
2017-05-12 发布于上海
举报
版权申诉

Prevalence of aac(6)-Ib-cr plasmid-mediated and chromosome-encoded fluoroquinolone resistance in Enterobacteriaceae in Italy 英文参考文献.doc

1、本文档共5页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Prevalence of aac(6)-Ib-cr plasmid-mediated and chromosome-encoded fluoroquinolone resistance in Enterobacteriaceae in Italy 英文参考文献

Frassonetal.GutPathogens2011,3:12 /content/3/1/12 SHORT REPORT OpenAccess Prevalenceofaac(6’)-Ib-crplasmid-mediatedand chromosome-encodedfluoroquinoloneresistance inEnterobacteriaceaeinItaly IlariaFrasson1,AntoniettaCavallaro2,CristinaBergo2,SaraNRichter1,2* andGiorgioPalù1,2 Abstract Thespreadofaac(6’)-Ib-crplasmid-mediatedquinoloneresistancedeterminantswasevaluatedin197 enterobacterialisolatesrecoveredinanItalianteachinghospital.Theaac(6’)-Ib-crgenewasfoundexclusivelyin Escherichiacolistrains.ThegenewaslocatedonaplasmidwhichpresentedadditionalESBLgenes.Mostofthe clinicalstrainswereclonallyrelatedanddisplayedthreepointmutationsatthetopoisomeraselevelwhich conferredhighresistancetofluoroquinolones. Keywords:aac(6’)-Ib-cr,fluoroquinolones,plasmid-mediatedresistance,Gram-negative Findings thewhole range ofMICofciprofloxacin; inparticular, Theaminoglycoside acetyltransferase Aac(6’)-Ib-cr var- 104samplesweresusceptible(MIC≤1),35intermediate iant, an enzyme usually encoded by a plasmid-borne (1MIC4),and58resistant(MIC≥4)tociprofloxa- gene,extendsitsdrugtargetstoincludefluoroquinolones cin.ExactMICvaluesweremeasuredbymeansofE-test (FQs)inadditiontoaminoglycosides.Itischaracterized strips (AB Biodisk, Solna, Sweden). Bacterial isolates by amino acid changes at codon 102 (Trp?Arg) and were:145E.coli,38K.pneumoniae,5Proteusmirabilis, codon179(Asp?Tyr).TheAac(6’)-Ib-crproteinisable 5Enterobacter aerogenes,2Enterobacter cloacae and2 tospecificallyacetylatehydrophilicFQspresentingafree Citrobacter freundii. Sample identity and results are piperazinylamine(i.e.ciprofloxacinandnorfloxacin)[1]. reportedinTable1. Theaac(6’)-Ib-crgenehasspreadrapidlyamongEnter- The presence of the aac(6’)-Ib or aac(6’)-Ib-cr genes obacteriaceae,andalthoughonlyconferring alow-level was assessed by PCR amplification and subsequent resistance, itmaycreateanenvironmentfacilitating the sequencing with primers: aacF 5’-ATGACTGAGCAT- selectionofmorehighlyresistantdete