Progressive Polycomb Assembly on H3K27me3 Compartments Generates Polycomb Bodies with Developmentally Regulated Motion 英文参考文献.docVIP

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Progressive Polycomb Assembly on H3K27me3 Compartments Generates Polycomb Bodies with Developmentally Regulated Motion 英文参考文献.doc

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Progressive Polycomb Assembly on H3K27me3 Compartments Generates Polycomb Bodies with Developmentally Regulated Motion 英文参考文献

ProgressivePolycombAssemblyonH3K27me3 CompartmentsGeneratesPolycombBodieswith DevelopmentallyRegulatedMotion ThierryCheutin,GiacomoCavalli* InstitutdeGe′ne′tiqueHumaine,CNRSUPR1142,Montpellier,France Abstract Polycomb group (PcG) proteins are conserved chromatin factors that maintain silencing of key developmental genes outsideoftheirexpressiondomains.Recentgenome-wideanalysesshowedaPolycomb(PC)distributionwithbindingto discrete PcG response elements (PREs). Within the cell nucleus, PcG proteins localize in structures called PC bodies that containPcG-silencedgenes,andithasbeenrecentlyshownthatPREsformlocalandlong-rangespatialnetworks.Here,we studied the nuclear distribution of two PcG proteins, PC and Polyhomeotic (PH). Thanks to a combination of immunostaining,immuno-FISH,andliveimagingofGFPfusionproteins,wecouldanalyzetheformationandthemobilityof PCbodiesduringflyembryogenesisaswellascomparetheirbehaviortothatofthecondensedfractionofeuchromatin. Immuno-FISH experiments show that PC bodies mainly correspond to 3D structural counterparts of the linear genomic domainsidentifiedingenome-widestudies.Duringearlyembryogenesis,PCandPHprogressivelyaccumulatewithinPC bodies,whichformnuclearstructureslocalizedondistincteuchromatindomainscontaininghistoneH3tri-methylatedon K27. Time-lapse analysis indicates that two types of motion influence the displacement of PC bodies and chromatin domainscontainingH2Av-GFP.First,chromatindomainsandPCbodiescoordinatelyundergolong-rangemotionsthatmay correspondtothemovementofwholechromosometerritories.Second,eachPCbodyandchromatindomainhasitsown fastandhighlyconstrained motion.Inthis motionregime,PCbodies movewithin volumesslightlylargerthanthoseof condensedchromatindomains.Moreover,bothtypesofdomainsmovewithinvolumesmuchsmallerthanchromosome territories,stronglyrestrictingtheirpossibilityofinteractionwithothernuclearstructures.ThefastmotionofPCbodiesand chromatin domains observed during early embryogenesis strongly decreases in lat