Protein Analysis-on-Chip Systems in Foodomics 英文参考文献.docVIP

Nutrients 2012, 4, 1475-1489; doi:10.3390/nu4101475 OPEN ACCESS nutrients ISSN 2072-6643 /journal/nutrients Review Protein Analysis-on-Chip Systems in Foodomics Filomena Nazzaro 1,*, Pierangelo Orlando 2, Florinda Fratianni 1, Aldo Di Luccia 1,3 and Raffaele Coppola 1 1 Institute of Food Science, ISA-CNR, Via Roma 64, Avellino 83100, Italy; E-Mails: fratianni@r.it (F.F.); a.diluccia@unifg.it (A.D.L.); direttore@r.it (R.C.) 2 Institute of Protein Biochemistry, IBP-CNR, Via P. Castellino 121, Napoli 80124, Italy; E-Mail: p.orlando@r.it 3 Department of Food Science, University of Foggia, Via Napoli 25, Foggia 71100, Italy * Author to whom correspondence should be addressed; E-Mail: mena@r.it; Tel.:+39-0825299102; Fax: +39-0825781585. Received: 22 May 2012; in revised form: 21 July 2012 / Accepted: 30 September 2012 / Published: 16 October 2012 Abstract: Protein compositional data can address nutritional, packaging, origin/authenticity, processing history, safety and other quality questions. Such data has been time-consuming and expensive to generate until recently but “protein analysis on a chip” systems are now available that can analyze a complex food sample in a few minutes and do not require great protein analytical expertise. We review some of the main new approaches with examples of their application and discuss their advantages and disadvantages. Keywords: lab-on-chip; protein; microisoelectrofocusing; proteomic; food 1. Protein Analysis and Lab-on-Chip Method Highlights: Faster and finer approaches for the analysis of food proteins are necessary for the assurance of food quality and safety, as well as for consumer health; lab-on-chip electrophoresis tries to overcome the difficulties of protein analysis, offering a micro-platform for a quick analysis of all proteins present in a food matrix beyond the confines of a traditional laboratory. Proteins are one of the fundamental constituents in many varieties of food