Proteomic Analyses of Nucleoid-Associated Proteins in Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus 英文参考文献.docVIP

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Proteomic Analyses of Nucleoid-Associated Proteins in Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus 英文参考文献.doc

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Proteomic Analyses of Nucleoid-Associated Proteins in Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus 英文参考文献

ProteomicAnalysesofNucleoid-AssociatedProteinsin Escherichiacoli,Pseudomonasaeruginosa,Bacillus subtilis,andStaphylococcusaureus RyosukeL.Ohniwa*,YuriUshijima,ShinjiSaito,KazuyaMorikawa InstituteofBasicMedicalSciences,GraduateSchoolofComprehensiveHumanSciences,UniversityofTsukuba,Tennodai,Tsukuba,Ibaraki,Japan Abstract Background: The bacterial nucleoid contains several hundred kinds of nucleoid-associated proteins (NAPs), which play criticalrolesingenomefunctionssuchastranscriptionandreplication.SeveralNAPs,suchasHuandH-NSinEscherichiacoli, havesofarbeenidentified. Methodology/PrincipalFindings:Log-andstationary-phasecellsofE.coli,Pseudomonasaeruginosa,Bacillussubtilis ,and Staphylococcusaureuswerelysedinspermidinesolutions.Nucleoidswerecollectedbysucrosegradientcentrifugation,and theirproteinconstituentsanalyzedbyliquidchromatography-massspectrometry/massspectrometry(LC-MS/MS).Over200 proteinswereidentifiedineachspecies.Envelopeandsolubleproteinfractionswerealsoidentified.Byusingthesedata sets,weobtainedlistsofcontaminant-subtractedproteinsenrichedinthenucleoidfractions(csNAPlists).Thelistsdonot coveralloftheNAPs,butincludedHuregardless ofthegrowthphasesandspecies.Inaddition,thecsNAPlistsofeach species suggested that the bacterial nucleoid is equipped with the species-specific set of global regulators, oxidation- reduction enzymes, and fatty acid synthases. This implies bacteria individually developed nucleoid associated proteins towardobtainingsimilarcharacteristics. Conclusions/Significance:OursisthefirststudytorevealhundredsofNAPsinthebacterialnucleoid,andtheobtaineddata set enabled us to overview some important features of the nucleoid. Several implications obtained from the present proteomicstudymaymakeitalandmarkforthefuturefunctionalandevolutionarystudyofthebacterialnucleoid. Citation: OhniwaRL,UshijimaY,SaitoS,MorikawaK(2011)ProteomicAnalysesofNucleoid-Associated Proteinsin Escherichia coli,Pseudomonas aeruginosa, Bacillussubtilis,andStaphyloco