Proteomic Analysis of the Differential Protein Expression Reveals Nuclear GAPDH in Activated T Lymphocytes 英文参考文献.docVIP

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Proteomic Analysis of the Differential Protein Expression Reveals Nuclear GAPDH in Activated T Lymphocytes 英文参考文献.doc

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Proteomic Analysis of the Differential Protein Expression Reveals Nuclear GAPDH in Activated T Lymphocytes 英文参考文献

ProteomicAnalysisoftheDifferentialProteinExpression RevealsNuclearGAPDHinActivatedTLymphocytes Wei-YunSheng¤a,Tzu-ChienV.Wang* DepartmentofMolecularandCellularBiology,ChangGungUniversity,Kwei-San,Taiwan Abstract Despite the important role of T cell activation in the adaptive immunity, very little is known about the functions of proteinsthataredifferentially expressedintheactivated Tcells.Inthisstudy,wehaveemployedproteomic approachto studythedifferentially expressed proteins inactivated Tcells. Atotal of25proteins wascharacterized thatdisplayed a decreased expression, while a total of 20 proteins was characterized that displayed an increased expression in the activatedTcells.Amongthem,glyceraldehyde-3-phosphate dehydrogenase (GAPDH)wasidentifiedunexpectedly asone of the up-regulated proteins. Western blot analysis of proteins separated by 2-dimensional gel electrophoresis had identified several modified GAPDHswhichweredetectable onlyintheactivated Tcells, butnotinresting Tcells. These modified GAPDHs had higher molecular mass and more basic PI, and were present in the nucleus ofactivated Tcells. Promoteroccupancystudiesbychromatinimmunoprecipitation assayrevealedthatnuclearGAPDHcouldbedetectedin thepromoter ofgenesthatwereup-regulated during Tcellactivation, butnotinthepromoter ofgenesthatwerenot unaffected or down-regulated. Our results suggest that nuclear GAPDH may function as transcriptional regulator in activated Tcells. Citation: Sheng W-Y, Wang T-CV (2009) Proteomic Analysis of the Differential Protein Expression Reveals Nuclear GAPDH in Activated T Lymphocytes. PLoS ONE4(7):e6322.doi:10.1371/journal.pone.0006322 Editor:JohanK.Sandberg,KarolinskaInstitutet,Sweden ReceivedApril8,2009;AcceptedJune15,2009;PublishedJuly21,2009 Copyright: ? 2009 Sheng, Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheorig