Proteomic characterization of iron deficiency responses in Cucumis sativus L. roots 英文参考文献.docVIP
- 1、本文档共15页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Proteomic characterization of iron deficiency responses in Cucumis sativus L. roots 英文参考文献
Donninietal.BMCPlantBiology2010,10:268
/1471-2229/10/268
RESEARCH ARTICLE
OpenAccess
Proteomiccharacterizationofirondeficiency
responsesinCucumissativusL.roots
SilviaDonnini,BhaktiPrinsi,AlfredoSNegri,GianpieroVigani,LucaEspen,GrazianoZocchi*
Abstract
Background:IrondeficiencyinducesinStrategyIplantsphysiological,biochemicalandmolecularmodifications
capabletoincreaseironuptakefromtherhizosphere.Thiseffortneedsareorganizationofmetabolicpathwaysto
efficientlysustainactivitieslinkedtotheacquisitionofiron;infact,carbohydratesandtheenergeticmetabolism
hasbeenshowntobeinvolvedintheseresponses.Theaimofthisworkwastofindbothaconfirmationofthe
alreadyexpectedchangeintheenzymeconcentrationsinducedincucumberroottissueinresponsetoiron
deficiencyaswellastofindnewinsightsontheinvolvementofotherpathways.
Results:Theproteomepatternofsolublecytosolicproteinsextractedfromrootswasobtainedby2-DE.Ofabout
twothousandspotsfound,onlythoseshowingatleastatwo-foldincreaseordecreaseintheconcentrationwere
consideredforsubsequentidentificationbymassspectrometry.Fifty-sevenproteinsshowedsignificantchanges,
and44ofthemwereidentified.Twenty-oneofthemwereincreasedinquantity,whereas23weredecreasedin
quantity.Mostoftheincreasedproteinsbelongtoglycolysisandnitrogenmetabolisminagreementwiththe
biochemicalevidence.Ontheotherhand,theproteinsbeingdecreasedbelongtothemetabolismofsucroseand
complexstructuralcarbohydratesandtostructuralproteins.
Conclusions:Thenewavailabletechniquesallowtocastnewlightonthemechanismsinvolvedinthechanges
occurringinplantsunderirondeficiency.Thedataobtainedfromthisproteomicstudyconfirmthemetabolic
changesoccurringincucumberasaresponsetoFedeficiency.Twomainconclusionsmaybedrawn.Thefirstone
istheconfirmationoftheincreaseintheglycolyticfluxandintheanaerobicmetabolismtosustaintheenergetic
efforttheFe-deficientplantsmustundertake.Thesecondconclusionis,ononehand,thedecreaseintheamount
ofenzymeslinkedtothebiosynthesisofcomplexcarbohydratesofthecellwall,and,ontheotherhand,the
increase
您可能关注的文档
- Protein Glycosylation in Helicobacter pylori Beyond the Flagellins 英文参考文献.doc
- Protein interactions in human genetic diseases 英文参考文献.doc
- Protein Kinase Cδ Stimulates Proteasome-Dependent Degradation of CEBPα during Apoptosis Induction of Leukemic Cells 英文参考文献.doc
- Protein Kinase C Delta (PKCδ) Affects Proliferation of Insulin-Secreting Cells by Promoting Nuclear Extrusion of the Cell Cycle Inhibitor p21Cip1WAF1 英文参考文献.doc
- Protein Kinases and Plant Pores 英文参考文献.doc
- Protein Kinase C-Dependent Dephosphorylation of Tyrosine Hydroxylase Requires the B56δ Heterotrimeric Form of Protein Phosphatase 2A 英文参考文献.doc
- Protein Localization with Flexible DNA or RNA 英文参考文献.doc
- Protein Kinase A Activity and Anchoring Are Required for Ovarian Cancer Cell Migration and Invasion 英文参考文献.doc
- Protein Meta-Functional Signatures from Combining Sequence, Structure, Evolution, and Amino Acid Property Information 英文参考文献.doc
- Protein Misfolding as an Underlying Molecular Defect in Mucopolysaccharidosis III Type C 英文参考文献.doc
- Proteomic Detection of Non-Annotated Protein-Coding Genes in Pseudomonas fluorescens Pf0-1 英文参考文献.doc
- Proteomic Identification of IPSEalpha-1 as a Major Hepatotoxin Secreted by Schistosoma mansoni Eggs 英文参考文献.doc
- Proteomic Analysis Shows Synthetic Oleanane Triterpenoid Binds to mTOR 英文参考文献.doc
- Proteomic Identification of S-Nitrosylated Golgi Proteins New Insights into Endothelial Cell Regulation by eNOS-Derived NO 英文参考文献.doc
- Proteomic Interrogation of Human Chromatin 英文参考文献.doc
- Proteomic Profiling of a Mouse Model for Ovarian Granulosa Cell Tumor Identifies VCP as a Highly Sensitive Serum Tumor Marker in Several Human Cancers 英文参考文献.doc
- Proteomic Comparison of Entamoeba histolytica and Entamoeba dispar and the Role of E. histolytica Alcohol Dehydrogenase 3 in Virulence 英文参考文献.doc
- Proteomic patterns analysis with multivariate calculations as a promising tool for prompt differentiation of early stage lung tissue with cancer and unchanged tissue material 英文参考文献.doc
- Proteomic Profiling of the Amniotic Fluid to Detect Inflammation, Infection, and Neonatal Sepsis 英文参考文献.doc
- Proteomic Interrogation of Androgen Action in Prostate Cancer Cells Reveals Roles of Aminoacyl tRNA Synthetases 英文参考文献.doc
文档评论(0)