Proteomic Studies of a Single CNS Synapse Type The Parallel FiberPurkinje Cell Synapse 英文参考文献.docVIP
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Proteomic Studies of a Single CNS Synapse Type The Parallel FiberPurkinje Cell Synapse 英文参考文献
o
PL SBIOLOGY
ProteomicStudiesofaSingleCNS
SynapseType:TheParallel
Fiber/PurkinjeCellSynapse
Fekrije Selimi1,2,3,Ileana M. Cristea4,5,Elizabeth Heller1,Brian T.Chait4,Nathaniel Heintz1*
1LaboratoryofMolecularBiology,HowardHughesMedicalInstitute,TheRockefellerUniversity,NewYork,NewYork,UnitedStatesofAmerica,2CNRS,UMR7102,Paris,
France,3UPMC,UMR7102,Paris,France,4LaboratoryforMassSpectrometryandGaseousIonChemistry,TheRockefellerUniversity,NewYork,NewYork,UnitedStatesof
America,5DepartmentofMolecularBiology,PrincetonUniversity,Princeton,NewJersey,UnitedStatesofAmerica
Precise neuronal networks underlie normal brain function and require distinct classes of synaptic connections.
Although it has been shown that certain individual proteins can localize to different classes of synapses, the
biochemical composition of specific synapse types is not known. Here, we have used a combination of genetically
engineered mice, affinity purification, and mass spectrometry to profile proteins at parallel fiber/Purkinje cell
synapses. We identify approximately 60 candidate postsynaptic proteins that can be classified into 11 functional
categories.Proteinsinvolvedinphospholipidmetabolismandsignaling,suchastheproteinkinaseMRCKc,aremajor
unrecognized components of this synapse type. We demonstrate that MRCKc can modulate maturation of dendritic
spinesinculturedcorticalneurons,andthatitislocalizedspecificallytoparallelfiber/Purkinjecellsynapsesinvivo.
Ourdataidentifyanovelsynapse-specificsignalingpathway,andprovideanapproachfordetailedinvestigationsof
thebiochemicalcomplexityofcentralnervoussystemsynapsetypes.
Citation: Selimi F, Cristea IM, Heller E, Chait BT, Heintz N (2009) Proteomic studies of a single CNS synapse type: The parallel fiber/Purkinje cell synapse. PLoS Biol 7(4):
e1000083.doi:10.1371/journal.pbio.1000083
16].Theseproteinshaveawiderangeoffunctions:receptors
toneurotransmitters,scaffoldproteins,kinases,enzymes,etc.
Introduction
Eachofthethousandsofcelltypespresentinthene
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