Pseudomonas aeruginosa Keratitis in Mice Effects of Topical Bacteriophage KPP12 Administration 英文参考文献.docVIP

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Pseudomonas aeruginosa Keratitis in Mice Effects of Topical Bacteriophage KPP12 Administration 英文参考文献.doc

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Pseudomonas aeruginosa Keratitis in Mice Effects of Topical Bacteriophage KPP12 Administration 英文参考文献

PseudomonasaeruginosaKeratitisinMice:Effectsof TopicalBacteriophageKPP12Administration KenFukuda1*,WakaIshida1,JumpeiUchiyama2,MohammadRashel2,Shin-ichiroKato3,TamaeMorita4, AsakoMuraoka5,TamakiSumi1,ShigenobuMatsuzaki2,MasanoriDaibata2,AtsukiFukushima1 1DepartmentofOphthalmologyandVisualScience,KochiMedicalSchool,Kochi,Japan,2DepartmentofMicrobiologyandInfection,KochiMedicalSchool,Kochi,Japan, 3ResearchInstituteofMolecularGenetics,KochiUniversity,Kochi,Japan,4KochiMedicalSchoolHospital,Kochi,Japan,5KochiGakuenJuniorCollege,Kochi,Japan Abstract The therapeutic effects of bacteriophage (phage) KPP12 in Pseudomonas aeruginosa keratitis were investigated in mice. MorphologicalanalysisshowedthatphageKPP12isamemberofthefamilyMyoviridae,morphotypeA1,andDNAsequence analysisrevealedthatphageKPP12issimilartoPB1-likeviruses.AnalysisofthephageKPP12genomedidnotidentifyany genes related to drug resistance, pathogenicity or lysogenicity, and so phage KPP12 may be a good candidate for therapeutic. KPP12 showed a broad host range for P. aeruginosa strains isolated from clinical ophthalmic infections. InoculationofthescarifiedcorneawithP.aeruginosacausedseverekeratitisandeventualcornealperforation.Subsequent single-dose administration of KPP12 eye-drops significantly improved disease outcome, and preserved the structural integrityandtransparencyoftheinfectedcornea.KPP12treatmentresultedinthesuppressionofneutrophilinfiltrationand greatlyenhancedbacterialclearanceintheinfectedcornea.Theseresultsindicatethatbacteriophageeye-dropsmaybe anoveladjunctiveoralternativetherapeuticagentforthetreatmentofinfectiouskeratitissecondarytoantibiotic-resistant bacteria. Citation: Fukuda K, Ishida W, Uchiyama J, Rashel M, Kato S-i, et al. (2012) Pseudomonas aeruginosa Keratitis in Mice: Effects of Topical Bacteriophage KPP12 Administration.PLoSONE7(10):e47742.doi:10.1371/journal.pone.0047742 Editor:MarkusM.Heimesaat,Charite′,CampusBenjaminFranklin,Germany ReceivedJune29,2012;AcceptedSeptember17,2012