Pseudomonas aeruginosa Population Structure Revisited 英文参考文献.docVIP

PseudomonasaeruginosaPopulationStructureRevisited Jean-PaulPirnay1*,FlorenceBilocq1,BrunoPot2,PierreCornelis3,MartinZizi4,JohanVanEldere5 ,Pieter Deschaght6,MarioVaneechoutte6,SergeJennes1,TyronePitt7,DanielDeVos1,3 1Laboratory for Molecular and Cellular Technology, Burn Centre, Queen Astrid Military Hospital, Brussel, Belgium, 2Applied Maths, Sint-Martens-Latem, Belgium, 3Laboratory ofMicrobial Interactions, Department ofMolecular andCellular Interactions,FlandersInteruniversity Institute ofBiotechnology, VrijeUniversiteit Brussel, Brussel, Belgium, 4Department of Physiology, Vrije Universiteit Brussel, Brussel, Belgium, 5Rega Institute and Laboratory of Microbiology, Gasthuisberg University Hospital, Catholic University of Leuven, Leuven, Belgium, 6Laboratory for Bacteriology Research, Department of Chemistry, Microbiology and Immunology, Ghent University,Ghent,Belgium,7LaboratoryofHealthcareAssociatedInfection,SpecialistandReferenceMicrobiologyDivision,HealthProtectionAgency,London,United Kingdom Abstract At present there are strong indications that Pseudomonas aeruginosa exhibits an epidemic population structure; clinical isolates are indistinguishable from environmental isolates, and they do not exhibit a specific (disease) habitat selection. However, some important issues, such as the worldwide emergence of highly transmissible P. aeruginosa clones among cysticfibrosis(CF)patientsandthespreadandpersistenceofmultidrugresistant(MDR)strainsinhospitalwardswithhigh antibioticpressure,remaincontentious.TofurtherinvestigatethepopulationstructureofP.aeruginosa,eightparameters wereanalyzedandcombinedfor328unrelatedisolates,collectedoverthelast125yearsfrom69localitiesin30countries onfivecontinents,fromdiverseclinical(humanandanimal)andenvironmentalhabitats.Theanalysedparameterswere:i)O serotype, ii) Fluorescent Amplified-Fragment Length Polymorphism (FALFP) pattern, nucleotide sequences of outer membraneproteingenes,iii)oprI,iv)oprL,v)oprD,vi)pyoverdinereceptorgenepro