Purified Mesenchymal Stem Cells Are an Efficient Source for iPS Cell Induction 英文参考文献.docVIP

下载本文档

9
0
约3.37万字
约 9页
2017-05-12 发布于上海
举报
版权申诉

Purified Mesenchymal Stem Cells Are an Efficient Source for iPS Cell Induction 英文参考文献.doc

1、本文档共9页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Purified Mesenchymal Stem Cells Are an Efficient Source for iPS Cell Induction 英文参考文献

PurifiedMesenchymalStemCellsAreanEfficientSource foriPSCellInduction KunimichiNiibe1,2,YoshimiKawamura1,DaisukeAraki1,2,SatoruMorikawa2,KyokoMiura1 ,Sadafumi Suzuki1,ShigetoShimmura3,TakehikoSunabori1,YoMabuchi1,YasuoNagai1,TaneakiNakagawa2, HideyukiOkano1,YumiMatsuzaki1* 1DepartmentofPhysiology,KeioUniversitySchoolofMedicine,Tokyo,Japan,2DepartmentofDentistryandOralSurgery,KeioUniversitySchoolofMedicine,Tokyo, Japan,3DepartmentofOphthalmology,KeioUniversitySchoolofMedicine,Tokyo,Japan Abstract Background: Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by the forced expressionofdefinedtranscriptionfactors.Althoughmostsomaticcellsarecapableofacquiringpluripotencywithminimal gene transduction, the poor efficiency of cell reprogramming and the uneven quality of iPS cells are still important problems.Inparticular,thechoiceofcelltypemostsuitableforinducinghigh-qualityiPScellsremainsunclear. Methodology/PrincipalFindings:Here,wegeneratediPScellsfromPDGFRa+Sca-1+(PaS)adultmousemesenchymalstem cells (MSCs) and PDGFRa2 Sca-12 osteo-progenitors (OP cells), and compared the induction efficiency and quality of individualiPSclones.MSCshadahigherreprogrammingefficiencycomparedwithOPcellsandTailTipFibroblasts(TTFs). The iPS cells induced from MSCs by Oct3/4, Sox2, and Klf4 appeared to be the closest equivalent to ES cells by DNA microarraygeneprofileandgermline-transmissionefficiency. Conclusions/Significance: Our findings suggest that a purified source of undifferentiated cells from adult tissue can produce high-quality iPS cells. In this context, prospectively enriched MSCs are a promising candidate for the efficient generationofhigh-qualityiPScells. Citation:NiibeK,KawamuraY,ArakiD,MorikawaS,MiuraK,etal.(2011)PurifiedMesenchymalStemCellsAreanEfficientSourceforiPSCellInduction.PLoS ONE6(3):e17610.doi:10.1371/journal.pone.0017610 Editor:ElianaAbdelhay,InstitutoNacionaldeCa?ncer, Brazil ReceivedJanuary6,2011;AcceptedJanuary31,2011;Publis