Quantitative X-ray Tomography of the Mouse Cochlea 英文参考文献.docVIP

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Quantitative X-ray Tomography of the Mouse Cochlea 英文参考文献.doc

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Quantitative X-ray Tomography of the Mouse Cochlea 英文参考文献

QuantitativeX-rayTomographyoftheMouseCochlea ChristophRau1,2,MargaretHwang2,Wah-KeatLee3,Claus-PeterRichter2,4,5* 1Diamond Light Source Ltd., Diamond House, Harwell Science and Innovation Campus, Didcot, United Kingdom, 2Department of Otolaryngology, Northwestern UniversityFeinbergSchoolofMedicine,Chicago,Illinois,UnitedStatesofAmerica,3AdvancedPhotonSource,ArgonneNationalLaboratory,Argonne,Illinois,United States of America, 4Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, 5The Hugh Knowles Center, DepartmentofCommunicationSciencesandDisorders,NorthwesternUniversity,Evanston,Illinois,UnitedStatesofAmerica Abstract ImagingwithhardX-raysallowsvisualizingcochlearstructureswhilemaintainingintrinsicqualitiesofthetissue,including structureandsize.WithcoherentX-rays,softtissues,includingmembranes,canbeimagedaswellascellsmakinguseofthe so-called in-line phase contrast. In the present experiments, partially coherent synchrotron radiation has been used for micro-tomography.Three-dimensionalreconstructionsofthemousecochleahavebeencreatedusingtheEM3Dsoftware andthevolumehasbeensegmentedintheAmiraSoftwareSuite.Thestructuresthathavebeenreconstructedincludescala tympani, scala media, scala vestibuli, Reissner’s membrane, basilar membrane, tectorial membrane, organ of Corti, spiral limbus,spiralganglionandcochlearnerve.Cross-sectionalareasofthescalaeweremeasured.Theresultsprovidearealistic andquantitativereconstructionofthecochlea. Citation: Rau C, Hwang M, Lee W-K, Richter C-P (2012) Quantitative X-ray Tomography of the Mouse Cochlea. PLoS ONE 7(4): e33568. doi:10.1371/ journal.pone.0033568 Editor:HaniA.Awad,UniversityofRochester,UnitedStatesofAmerica ReceivedDecember5,2011;AcceptedFebruary13,2012;PublishedApril2,2012 Thisisanopen-accessarticle,freeofallcopyright,andmaybefreelyreproduced,distributed,transmitted,modified,builtupon,orotherwiseusedbyanyonefor anylawfulpurpose.TheworkismadeavailableundertheCreativeCommonsC