Quorum Quenching Bacillus sonorensis Isolated from Soya Sauce Fermentation Brine 英文参考文献.docVIP
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Quorum Quenching Bacillus sonorensis Isolated from Soya Sauce Fermentation Brine 英文参考文献
Sensors 2012, 12, 4065-4073; doi:10.3390/s120404065
OPEN ACCESS
sensors
ISSN 1424-8220
/journal/sensors
Article
Quorum Quenching Bacillus sonorensis Isolated from Soya Sauce
Fermentation Brine
Wai-Fong Yin 1, Hun-Jiat Tung 1, Choon-Kook Sam 2, Chong-Lek Koh 2 and Kok-Gan Chan 1,*
1
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science,
University of Malaya, 50603 Kuala Lumpur, Malaysia;
E-Mails: yinwaifong@ (W.-F.Y.); jiatth@ (H.-J.T.)
2
Natural Sciences and Science Education AG, National Institute of Education,
Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore;
E-Mails: choonkook.sam@.sg (C.-K.S.); chonglek.koh@.sg (C.-L.K.)
* Author to whom correspondence should be addressed; E-Mail: kokgan@.my;
Tel.: +603-7967-5162; Fax: +603-7967-4509.
Received: 16 January 2012; in revised form: 6 March 2012 / Accepted: 22 March 2012 /
Published: 27 March 2012
Abstract: An N-acylhomoserine lactone (AHL)-degrading bacterial strain, L62, was
isolated from a sample of fermentation brine of Chinese soya sauce by using rich medium
agar supplemented with soya sauce (10% v/v). L62, a rod-shaped Gram positive bacterium
with amylolytic activity, was phylogentically related to Bacillus sonorensis by 16S ribosomal
DNA and rpoB sequence analyses. B. sonorensis L62 efficiently degraded N-3-oxohexanoyl
homoserine lactone and N-octanoylhomoserine lactone. However, the aiiA homologue,
encoding an autoinducer inactivation enzyme catalyzing the degradation of AHLs, was not
detected in L62, suggesting the presence of a different AHL-degrading gene in L62. To the
best of our knowledge, this is the first report of AHL-degrading B. sonorensis from soya
sauce liquid state fermentation.
Keywords: Bacillus sonorensis; N-acylhomoserine lactone; quorum quenching; quorum
sensing; soya sauce fermentation brine
Sensors 2012, 12
4066
1. Introduction
Gram negative bacterial communication achie
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