Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing 英文参考文献.docVIP

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Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing 英文参考文献.doc

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Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing 英文参考文献

RapidScreeningofComplexDNASamplesbySingle- MoleculeAmplificationandSequencing JiaqiHuang1,ZongliZheng1,2*,AndersF.Andersson3,LarsEngstrand2,4,WeiminYe1 1Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden, 2Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden, 3Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden, 4Swedish Institute for CommunicableInfectiousDiseaseControl,Solna,Sweden Abstract MicrobialcloningmakesSangersequencingofcomplexDNAsamplespossiblebutislaborintensive.Wepresentasimple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing,althoughinalow-throughputmanner,fromsub-picogramquantitiesofDNA.Themethodcanalsobeusedfor quickqualitycontrolofnext-generationsequencinglibraries,aswasdemonstratedforametagenomicsample. Citation: Huang J, Zheng Z, Andersson AF, Engstrand L, Ye W (2011) Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing.PLoSONE6(5):e19723.doi:10.1371/journal.pone.0019723 Editor:JackAnthonyGilbert,ArgonneNationalLaboratory,UnitedStatesofAmerica ReceivedMarch18,2011;AcceptedApril8,2011;PublishedMay19,2011 Copyright: ? 2011 Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwassupportedbytheSixthResearchFrameworkProgramoftheEuropeanUnion,projectINCA(LSHC-CT-2005-018704)toOlofNyre′nandLE, andSwedishCancerSocietytoOlofNyre′n,WY,andLE.JHandZZwerealsosupportedbyscholarshipsfromtheKarolinskaInstitutet(KID).AAwasfundedbya grantfromtheSwedishResearchCouncilFORMAS.Thefundershadnoroleinstudydesign,datacollectionandanalysis,decisiontopublish,orpreparationofthe manuscript. CompetingInterests:Theauthorshavedeclaredthatno