Recovery and characterization of a Citrus clementina Hort. ex Tan. Clemenules haploid plant selected to establish the reference whole Citrus genome sequence 英文参考文献.docVIP

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Recovery and characterization of a Citrus clementina Hort. ex Tan. Clemenules haploid plant selected to establish the reference whole Citrus genome sequence 英文参考文献.doc

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Recovery and characterization of a Citrus clementina Hort. ex Tan. Clemenules haploid plant selected to establish the reference whole Citrus genome sequence 英文参考文献

BMC Plant Biology BioMedCentral Research article Open Access Recovery and characterization of a Citrus clementina Hort. ex Tan. Clemenules haploid plant selected to establish the reference whole Citrus genome sequence Pablo Aleza1, José Juárez1, María Hernández1, José A Pina1, Patrick Ollitrault2 and Luis Navarro*1 Address: 1Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain and 2Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France Email: Pablo Aleza - aleza@ivia.es; José Juárez - jjuarez@ivia.es; María Hernández - mariaher@ivia.es; José A Pina - japina@ivia.es; Patrick Ollitrault - patrick.ollitrault@cirad.fr; Luis Navarro* - lnavarro@ivia.es * Corresponding author Published: 22 August 2009 Received: 16 February 2009 Accepted: 22 August 2009 BMC Plant Biology 2009, 9:110 doi:10.1186/1471-2229-9-110 This article is available from: /1471-2229/9/110 ? 2009 Aleza et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: In recent years, the development of structural genomics has generated a growing interest in obtaining haploid plants. The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects. Commercial citrus species are characterized by high heterozygosity, making it difficult to assemble large genome sequences. Thus, the International Citrus Genomic Consortium (ICGC) decided to establish a reference whole citrus genome sequence from a homozygous plant. Due to the existence of important molecular resources and