Relationships between Levels of Serum IgE, Cell-Bound IgE, and IgE-Receptors on Peripheral Blood Cells in a Pediatric Population 英文参考文献.docVIP

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Relationships between Levels of Serum IgE, Cell-Bound IgE, and IgE-Receptors on Peripheral Blood Cells in a Pediatric Population 英文参考文献.doc

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Relationships between Levels of Serum IgE, Cell-Bound IgE, and IgE-Receptors on Peripheral Blood Cells in a Pediatric Population 英文参考文献

RelationshipsbetweenLevelsofSerumIgE,Cell-Bound IgE,andIgE-ReceptorsonPeripheralBloodCellsina PediatricPopulation EleonoraDehlink1,2,AlexandraH.Baker1,ElizabethYen1,SamuelNurko1.,EddaFiebiger1*. 1Division of Gastroenterology and Nutrition and EGID Centre, Children’s Hospital Boston, Harvard Medical School, Boston, Massachusetts, United States of America, 2DepartmentofPediatricsandAdolescentMedicine,MedicalUniversityofVienna,WaehringerGuertel,Vienna,Austria Abstract Background: Elevated serum immunoglobulin (Ig) E is a diagnostic marker of immediate-type allergic reactions. We hypothesize that serum IgE does not necessarily reflect total body IgE because in vivo IgE can be bound to cell surface receptorssuchasFceRIandFceRII(CD23).Theaimofthisstudywastoanalyzetherelationshipsbetweenlevelsofserum IgE,cell-boundIgE,andIgE-receptorsonperipheralbloodcellsinapediatricpopulation. Methodology:Wholebloodsamplesfrom48children(26boys,22girls,meanage10,365,4years)wereanalyzedbyflow cytometry for FceRI, CD23, and cell-bound IgE on dendritic cells (CD11c+MHC class II+), monocytes (CD14+ ), basophils (CD123+MHCclassII-)andneutrophils(myeloperoxidase+).TotalserumIgEwasmeasuredbyELISAandconvertedintoz- unitstoaccountforage-dependentnormalranges.CorrelationswerecalculatedusingSpearmanrankcorrelationtest. Principal Findings: Dendritic cells, monocytes, basophils, and neutrophils expressed the high affinity IgE-receptor FceRI. Dendritic cells and monocytes also expressed the low affinity receptor CD23. The majority of IgE-receptor positive cells carried IgE on their surface. Expression of both IgE receptors was tightly correlated with cell-bound IgE. In general, cell- boundIgEonFceRI+cellscorrelatedwellwithserumIgE.However,somepatientscarriedhighamountsofcell-boundIgE despitelowtotalserumIgElevels. Conclusion/Significance:Inpediatricpatients,levelsofage-adjustedserumIgE,cell-boundIgE,andFceRIcorrelate.Evenin theabsenceofelevatedlevelsofserumIgE,cell-boundIgEcanbedetectedonperipheralbl