Remote Ischemic Preconditioning (RIPC) Modifies Plasma Proteome in Humans 英文参考文献.docVIP

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Remote Ischemic Preconditioning (RIPC) Modifies Plasma Proteome in Humans 英文参考文献.doc

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Remote Ischemic Preconditioning (RIPC) Modifies Plasma Proteome in Humans 英文参考文献

RemoteIschemicPreconditioning(RIPC)ModifiesPlasma ProteomeinHumans MicheleHepponstall1,2,3,4,VeraIgnjatovic1,3,SteveBinos4,PaulMonagle1,3,BrynJones1,2 MichaelH.H.Cheung1,2,3,Yvesd’Udekem1,2,IgorE.Konstantinov1,2,3 , * 1Haematology Research, Murdoch Childrens Research Institute; Melbourne, Victoria, Australia, 2Cardiac Surgery Unit and Cardiology, Royal Children’s Hospital; Melbourne,Victoria, Australia,3DepartmentofPaediatrics,TheUniversityofMelbourne;Melbourne,Victoria, Australia, 4BioscienceResearchDivision,Departmentof PrimaryIndustries,Melbourne,Victoria,Australia Abstract Remote Ischemic Preconditioning (RIPC) induced by brief episodes of ischemia of the limb protects against multi-organ damagebyischemia-reperfusion(IR).AlthoughithasbeendemonstratedthatRIPCaffectsgeneexpression,theproteomic responsetoRIPChasnotbeendetermined.ThisstudyaimedtoexamineRIPCinducedchangesintheplasmaproteome. Five healthy adult volunteers had 4 cycles of 5min ischemia alternating with 5min reperfusion of the forearm. Blood samplesweretakenfromtheipsilateralarmpriortofirstischaemia,immediatelyaftereachepisodeofischemiaaswellas,at 15min and 24h after the last episode of ischemia. Plasma samples from five individuals were analysed using two complementary techniques. Individual samples were analysed using 2Dimensional Difference in gel electrophoresis (2D DIGE)andmassspectrometry(MS).Pooledsamplesforeachofthetime-pointsunderwenttrypsindigestionandpeptides generatedwereanalysedintriplicateusingLiquidChromatographyandMS(LC-MS).Sixproteinschangedinresponseto RIPC using 2D DIGE analysis, while 48 proteins were found to be differentially regulated using LC-MS. The proteins of interest were involved in acute phase response signalling, and physiological molecular and cellular functions. The RIPC stimulusmodifiestheplasmaproteincontentinbloodtakenfromtheischemicarminacumulativefashionandevokesa proteomicresponseinperipheralblood. Citation: Hepponstall M, Ignjatovic V, Binos S, Monagle P,