Respiratory Syncytial Virus Persistence in Macrophages Alters the Profile of Cellular Gene Expression 英文参考文献.docVIP

Viruses2012, 4, 3270-3280; doi:10.3390/v4123270 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Review Respiratory Syncytial Virus Persistence in Macrophages Alters the Profile of Cellular Gene Expression Evelyn Rivera-Toledo * and Beatríz Gómez Department of Microbiology and Parasitology, Faculty of Medicine, Universidad Nacional Autónoma de México, Circuito exterior s/n, Ciudad Universitaria, México D.F., C.P. 04510, Mexico; E-Mail: begomez@servidor.unam.mx * Author to whom correspondence should be addressed; E-Mail: evelynmicro@; Tel.: +52-55-5623-2469; Fax: +52-55-5623-2382. Received: 23 October 2012; in revised form: 14 November 2012 / Accepted: 15 November 2012 / Published: 22 November 2012 Abstract: Viruses can persistently infect differentiated cells through regulation of expression of both their own genes and those of the host cell, thereby evading detection by the host’s immune system and achieving residence in a non-lytic state. Models in vitro with cell lines are useful tools in understanding the mechanisms associated with the establishment of viral persistence. In particular, a model to study respiratory syncytial virus (RSV) persistence in a murine macrophage-like cell line has been established. Compared to non-infected macrophages, macrophages persistently infected with RSV show altered expression both of genes coding for cytokines and trans-membrane proteins associated with antigen uptake and of genes related to cell survival. The biological changes associated with altered gene expression in macrophages as a consequence of persistent RSV infection are summarized. Keywords: Respiratory syncytial virus; viral persistence; macrophages; P388D1; altered gene expression 1. The Virus: Characteristics, Pathogenesis, and Epidemiology Respiratory syncytial virus (RSV; family Paramyxoviridae, genus Pneumovirus) is a highly infectious agent—more so th