Response Surface Optimized Ultrasonic-Assisted Extraction of Flavonoids from Sparganii Rhizoma and Evaluation of Their in Vitro Antioxidant Activities 英文参考文献.docVIP

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Response Surface Optimized Ultrasonic-Assisted Extraction of Flavonoids from Sparganii Rhizoma and Evaluation of Their in Vitro Antioxidant Activities 英文参考文献.doc

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Response Surface Optimized Ultrasonic-Assisted Extraction of Flavonoids from Sparganii Rhizoma and Evaluation of Their in Vitro Antioxidant Activities 英文参考文献

Molecules 2012, 17, 6769-6783; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Response Surface Optimized Ultrasonic-Assisted Extraction of Flavonoids from Sparganii Rhizoma and Evaluation of Their in Vitro Antioxidant Activities Xinsheng Wang 1,2, Qinan Wu 1,3,*, Yanfang Wu 4, Guangyun Chen 1, Wei Yue 1 and Qiaoli Liang 1,3 1 Pharmaceutical School, Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu, China 2 Chemical and Pharmaceutical School, Henan University of Science and Technology, Luoyang 471003, Henan, China 3 Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu, China 4 Pharmaceutical School, Xinxiang Medical University, Xinxiang 453003, Henan, China * Author to whom correspondence should be addressed; E-Mail: qnlxw@; Tel.: +86-25-8581-1059; Fax: +86-25-8581-1060. Received: 16 April 2012; in revised form: 22 May 2012 / Accepted: 23 May 2012 / Published: 4 June 2012 Abstract: An efficient ultrasound-assisted extraction technique was employed to extract total flavonoids from Sparganii rhizoma. The optimum extraction conditions for the highest yield of total flavonoids were ethanol concentration 53.62%, ultrasonication time 29.41 min and ultrasound power 300 W, which were determined using response surface methodology. The extraction yields of the optimal ultrasound-assisted extraction were higher than using conventional extraction. The crude extract was then purified on a polyamide resin, whereby the flavonoids content in the purified extract increased to 94.62%. The antioxidant activities of the purified flavonoids including DPPH radical scavenging activity, ABTS+ radical scavenging activity, reducing power, hydroxyl radical scavenging activity and superoxide anion scavenging activity, were evaluated in vitro, which suggested that the flavonoids show