Response to Comments of Peter G. Mantle 英文参考文献.docVIP

Toxins 2010, 2, 2337-2339; doi:10.3390/toxins2102337 OPEN ACCESS toxins ISSN 2072-6651 /journal/toxins Reply Response to Comments of Peter G. Mantle Gary G. Schwartz 1,*, Richard A. Manderville 2 and Annie Pfohl-Leszkowicz 3 1 Departments of Cancer Biology, Urology, and Epidemiology and Prevention, Wake Forest University, Winston-Salem, NC 27157, USA 2 Department of Chemistry and Toxicology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada; E-Mail: rmanderv@uoguelph.ca 3 University of Toulouse, Laboratory Chemical Engineering, Department Bioprocess Microbial System, UMR CNRS/INPT/UPS 5503, ENSA Toulouse, 1 avenue de l’Agrobiop?le, BP 32607, 31326, Auzeville-Tolosane, France; E-Mail: leszkowicz@ensat.fr * Author to whom correspondence should be addressed; E-Mail: gschwart@; Tel.: +1-336-716-7446; Fax: +1-336-716-5687. Received: 17 September 2010 / Accepted: 28 September 2010 / Published: 29 September 2010 Abstract: The apparently high yield of testis tumors (25%) in rats exposed long-term to Ochratoxin A (OTA) is uninterpretable without data on tumor yield in unexposed rats. Conversely, our demonstration that prenatal exposure to OTA induces DNA adducts in the testes of newborn mice and the absence of these adducts in the testes of mice not exposed prenatally to OTA, is evidence for the presumptive carcinogenicity of OTA in the testis. Together with recent data showing that prenatal exposure to OTA depresses expression of DMRT1, a tumor suppressor gene in the testis, our findings suggest that OTA may be a cause of testicular cancer. Keywords: ochratoxin; testicular cancer; DNA adduct We thank Pr. Mantle for his comments about our paper [1]. In his commentary, Pr. Mantle refers to perceived mis-citations of his papers. In particular, we cited his recent observation that 6/24 (25%) of aged Fisher rats exposed to Ochratoxin A via the diet developed testicular tumors [2]. We interpreted this as a priori evidence