Resident Cardiac Immune Cells and Expression of the Ectonucleotidase Enzymes CD39 and CD73 after Ischemic Injury 英文参考文献.docVIP

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Resident Cardiac Immune Cells and Expression of the Ectonucleotidase Enzymes CD39 and CD73 after Ischemic Injury 英文参考文献.doc

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Resident Cardiac Immune Cells and Expression of the Ectonucleotidase Enzymes CD39 and CD73 after Ischemic Injury 英文参考文献

ResidentCardiacImmuneCellsandExpressionofthe EctonucleotidaseEnzymesCD39andCD73afterIschemic Injury FlorianBo¨nner.,NadineBorg.,SandraBurghoff,Ju¨rgenSchrader* DepartmentofMolecularCardiology,Heinrich-Heine-UniversityDu¨sseldorf,Du¨sseldorf,Germany Abstract Background: The ectoenzymes CD39 and CD73 are expressed by a broad range of immune cells and promote the extracellulardegradationofnucleotidestoanti-inflammatoryadenosine.ThisstudyexploredtheabundanceofCD73and CD39oncirculatingandresidentcardiacleukocytesandcoronaryendothelialcellsundercontrolconditionsandinresponse toinflammationfollowingmyocardialischemiaandreperfusion(I/R). Methods and Results: A method was elaborated to permit FACS analysis of non-myocardial cells (resident leukocytes, coronary endothelium and CD312 CD452 cells) of the unstressed heart. Under control conditions the murine heart contained2.3610 residentleukocytes/mgtissue,themostprominentfractionbeingantigen-presentingmononuclearcells (CD11b CD11c+F4/80+MHCII+)followedbyB-cells,monocytesandT-cells.CD73washighlyexpressedoncirculatingand resident cardiac lymphoid cells with little expression on myeloid cells, while the opposite was true for CD39. CardiomyocytesanderythrocytesdonotmeasurablyexpressCD39/CD73andCD39dominatesoncoronaryendothelium. ThreedaysafterI/R,CD73wassignificantlyupregulatedoninvadinggranulocytes(2.8-fold)andT-cells(1.5-fold).Compared withcoronaryendothelialcells,CD73associatedwithleukocytescomprised2/3ofthetotalcardiacCD73. 3 + Conclusion: Our study suggests that extracellular ATP formed during I/R is preferentially degraded by CD39 present on myeloidcells,whiletheformationofimmunosuppressiveadenosineismainlycatalysedbyCD73presentongranulocytes and lymphoid cells. Upregulated CD73 on granulocytes and T-cells infiltrating the injured heart is consistent with the existenceofanautocrineadenosinergicloopwhichmaypromotethehealingprocess. Citation:Bo¨nnerF,BorgN,BurghoffS,SchraderJ(2012)ResidentCardiacImmuneCellsandExpressionoft