Replication-Competent Recombinant Porcine Reproductive and Respiratory Syndrome (PRRS) Viruses Expressing Indicator Proteins and Antiviral Cytokines 英文参考文献.docVIP

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Replication-Competent Recombinant Porcine Reproductive and Respiratory Syndrome (PRRS) Viruses Expressing Indicator Proteins and Antiviral Cytokines 英文参考文献.doc

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Replication-Competent Recombinant Porcine Reproductive and Respiratory Syndrome (PRRS) Viruses Expressing Indicator Proteins and Antiviral Cytokines 英文参考文献

Viruses 2012, 4, 102-116; doi:10.3390/v4010102 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Article Replication-Competent Recombinant Porcine Reproductive and Respiratory Syndrome (PRRS) Viruses Expressing Indicator Proteins and Antiviral Cytokines Yongming Sang 1, Jishu Shi 1, Wenjing Sang 2, Raymond R. R. Rowland 2 and Frank Blecha 1,* 1 Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA; E-Mails: ysang@ (Y.S.); jshi@ (J.S.) 2 Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA; E-Mails: wenjing@ (W.S.); browland@ (R.R.R.R.) * Author to whom correspondence should be addressed; E-Mail: blecha@; Tel.: +1-785-532-4537; Fax: +1-785-532-4557. Received: 23 December 2011; in revised form: 10 January 2012 / Accepted: 14 January 2012 / Published: 18 January 2012 Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) can subvert early innate immunity, which leads to ineffective antimicrobial responses. Overcoming immune subversion is critical for developing vaccines and other measures to control this devastating swine virus. The overall goal of this work was to enhance innate and adaptive immunity following vaccination through the expression of interferon (IFN) genes by the PRRSV genome. We have constructed a series of recombinant PRRS viruses using an infectious PRRSV cDNA clone (pCMV-P129). Coding regions of exogenous genes, which included Renilla luciferase (Rluc), green and red fluorescent proteins (GFP and DsRed, respectively) and several interferons (IFNs), were constructed and expressed through a unique subgenomic mRNA placed between ORF1b and ORF2 of the PRRSV infectious clone. The constructs, which expressed Rluc, GFP, DsRed, efficiently produced progeny viruses and mimicked the parental virus in both MARC-145 cells and porcine