Removing Biofilms from Microstructured Titanium Ex Vivo A Novel Approach Using Atmospheric Plasma Technology 英文参考文献.docVIP

下载本文档

4
0
约4.26万字
约 9页
2017-05-13 发布于上海
举报
版权申诉

Removing Biofilms from Microstructured Titanium Ex Vivo A Novel Approach Using Atmospheric Plasma Technology 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Removing Biofilms from Microstructured Titanium Ex Vivo A Novel Approach Using Atmospheric Plasma Technology 英文参考文献

RemovingBiofilmsfromMicrostructuredTitaniumEx Vivo:ANovelApproachUsingAtmosphericPlasma Technology StefanRupf1*,AhmadNourIdlibi1,6,FuadAlMarrawi1,MatthiasHannig1,AndreasSchubert2,Lutzvon Mueller3,WolfgangSpitzer4,HenrikHoltmann4,AntjeLehmann5,AndreRueppell1,5,AxelSchindler5 1Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Saarland University Hospital, Homburg/Saar, Germany, 2Vascular Biology Group, Fraunhofer Institute, Leipzig, Germany, 3Department of Medical Microbiology, Saarland University Hospital, Homburg/Saar, Germany, 4Clinic for Oral and Maxillofacial Surgery, UniversityClinicofSaarland,Homburg/Saar,Germany,5LeibnizInstituteofSurfaceModification(IOM),Leipzig,Germany,6DepartmentofPeriodontology,Facultyof Dentistry,UniversityofAleppo,Aleppo,Syria Abstract The removal of biofilms from microstructured titanium used for dental implants is a still unresolved challenge. This experimentalstudyinvestigateddisinfection andremovalof insituformedbiofilms frommicrostructuredtitaniumusing coldatmosphericplasmaincombinationwithair/waterspray.Titaniumdiscs(roughness(Ra):1.96 mm)wereexposedto humanoralcavitiesfor24and72hours(n=149each)toproducebiofilms.Biofilmthicknesswasdeterminedusingconfocal laser scanning microscopy (n=5 each). Plasma treatment of biofilms was carried out ex vivo using a microwave-driven pulsedplasmasourceworkingattemperaturesfrom39to43uC.Followingplasmatreatment,onegroupwasair/waterspray treated before re-treatment by second plasma pulses. Vital microorganisms on the titanium surfaces were identified by contact culture (Rodac agar plates). Biofilm presence and bacterial viability were quantified by fluorescence microscopy. Morphologyoftitaniumsurfacesandattachedbiofilmswasvisualizedbyscanningelectronmicroscopy(SEM).Totalprotein amounts of biofilms were colorimetrically quantified. Untreated and air/water treated biofilms served as controls. Cold plasmatreatmentofnativebiofilmswithameanthicknessof19 mm(24h)to91mm