REVEILLE8 and PSEUDO-REPONSE REGULATOR5 Form a Negative Feedback Loop within the Arabidopsis Circadian Clock 英文参考文献.docVIP

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REVEILLE8 and PSEUDO-REPONSE REGULATOR5 Form a Negative Feedback Loop within the Arabidopsis Circadian Clock 英文参考文献.doc

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REVEILLE8 and PSEUDO-REPONSE REGULATOR5 Form a Negative Feedback Loop within the Arabidopsis Circadian Clock 英文参考文献

REVEILLE8andPSEUDO-REPONSEREGULATOR5Forma NegativeFeedbackLoopwithintheArabidopsis CircadianClock ReetikaRawat1,NozomuTakahashi1.,PollyYingshanHsu1.,MatthewA.Jones1,JacobSchwartz1, MichelleR.Salemi2,BrettS.Phinney2,StaceyL.Harmer1* 1DepartmentofPlantBiology,CollegeofBiologicalSciences,UniversityofCaliforniaDavis,Davis,California,UnitedStatesofAmerica,2GenomeCenter,ProteomicsCore, GenomeandBiomedicalSciencesFacility,UniversityofCaliforniaDavis,Davis,California,UnitedStatesofAmerica Abstract Circadian rhythms provide organisms with an adaptive advantage, allowing them to regulate physiological and developmentaleventssothattheyoccuratthemostappropriatetimeofday.Inplants,asinothereukaryotes,multiple transcriptionalfeedbackloopsarecentraltoclockfunction.Inonesuchfeedbackloop,theMyb-liketranscriptionfactors CCA1andLHYdirectlyrepressexpressionofthepseudoresponseregulatorTOC1bybindingtoaneveningelement(EE)in theTOC1promoter.AnotherkeyregulatorycircuitinvolvesCCA1andLHYandtheTOC1homologsPRR5,PRR7,andPRR9. PurificationofEE–bindingproteinsfromplantextractsfollowedbymassspectrometryledtotheidentificationofRVE8,a homologofCCA1andLHY.Similartothesewell-knownclockgenes,expressionofRVE8iscircadian-regulatedwithadawn phase of expression, and RVE8 binds specifically to the EE. However, whereas cca1 and lhy mutants have short period phenotypesandoverexpressionofeithergenecausesarrhythmia,rve8mutantshavelong-periodandRVE8-OXplantshave short-periodphenotypes.Lightinputtotheclockisnormalinrve8,buttemperaturecompensation(ahallmarkofcircadian rhythms)isperturbed.RVE8bindstothepromotersofbothTOC1andPRR5inthesubjectiveafternoon,butsurprisingly onlyPRR5expressionisperturbedbyoverexpressionofRVE8.Together,ourdataindicatethatRVE8promotesexpressionof asubsetofEE–containingclockgenestowardstheendofthesubjectivedayandformsanegativefeedbackloopwithPRR5. Thus RVE8 and its homologs CCA1 and LHY function close to the circadian oscillator but act via distinct molecular mechanisms. Citation: Rawat R,