Reverse Engineering of the Spindle Assembly Checkpoint 英文参考文献.docVIP

下载本文档

1
0
约5.25万字
约 11页
2017-05-13 发布于上海
举报
版权申诉

Reverse Engineering of the Spindle Assembly Checkpoint 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Reverse Engineering of the Spindle Assembly Checkpoint 英文参考文献

ReverseEngineeringoftheSpindleAssembly Checkpoint AndreasDoncic1,2,EshelBen-Jacob3,ShmuelEinav2,NaamaBarkai1,4* 1DepartmentofMolecularGenetics,WeizmannInstituteofScience,Rehovot,Israel,2DepartmentofBiomedicalEngineering,TheIbyandAladarFleischmanFacultyof Engineering,TelAvivUniversity,RamatAviv,Israel,3SchoolofPhysicsandAstronomy,BeverlyandRaymondSacklerFacultyofExactSciences,TelAvivUniversity,Tel Aviv,Israel,4DepartmentofPhysicsofComplexSystems,WeizmannInstituteofScience,Rehovot,Israel Abstract The Spindle Assembly Checkpoint (SAC) is an intracellular mechanism that ensures proper chromosome segregation. By inhibiting Cdc20, a co-factor of the Anaphase Promoting Complex (APC), the checkpoint arrests the cell cycle until all chromosomes are properly attached to the mitotic spindle. Inhibition of Cdc20 is mediated by a conserved network of interactingproteins.Theindividualfunctionsoftheseproteinsarewellcharacterized,butunderstandingoftheirintegrated functionisstillrudimentary.Weheredescribeourattemptstoreverse-engineertheSACnetworkbasedongenedeletion phenotypes.WebegunbyformulatingageneralmodeloftheSACwhichenablesustopredicttherateofchromosomal missegregationforanyputativesetofinteractionsbetweentheSACproteins.Nextthemissegregationratesofsevenyeast strains are measured in response to the deletion of one or two checkpoint proteins. Finally, we searched for the set of interactionsthatcorrectlypredictedtheobservedmissegregationratesofalldeletionmutants.Remarkably,althoughbased ononlysevenphenotypes,theconsistentnetworkweobtainedsuccessfullyreproducesmanyoftheknownpropertiesof theSAC.Furtherinsightsprovidedbyouranalysisarediscussed. Citation: Doncic A, Ben-Jacob E, Einav S, Barkai N (2009) Reverse Engineering of the Spindle Assembly Checkpoint. PLoS ONE 4(8): e6495. doi:10.1371/ journal.pone.0006495 Editor:RayaKhanin,UniversityofGlasgow,UnitedKingdom ReceivedFebruary22,2009;AcceptedJuly3,2009;PublishedAugust4,2009 Copyright: ? 2009 Doncic et al. This is an open-access a