Rheumatoid synovial fluid interleukin-17-producing CD4 T cells have abundant tumor necrosis factor-alpha co-expression, but little interleukin-22 and interleukin-23R expression 英文参考文献.docVIP

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Rheumatoid synovial fluid interleukin-17-producing CD4 T cells have abundant tumor necrosis factor-alpha co-expression, but little interleukin-22 and interleukin-23R expression 英文参考文献.doc

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Rheumatoid synovial fluid interleukin-17-producing CD4 T cells have abundant tumor necrosis factor-alpha co-expression, but little interleukin-22 and interleukin-23R expression 英文参考文献

Churchetal.ArthritisResearchTherapy2010,12:R184 /content/12/5/R184 RESEARCH ARTICLE OpenAccess Rheumatoidsynovialfluidinterleukin-17- producingCD4Tcellshaveabundanttumor necrosisfactor-alphaco-expression,butlittle interleukin-22andinterleukin-23Rexpression LeighDChurch1,AndrewDFiler1,2,EstherHidalgo1,KatherineAHowlett1,AndrewMCThomas3, StephenRapecki4,DagmarScheel-Toellner1,ChristopherDBuckley1,2,KarimRaza1,2* Abstract Introduction:Th17cellshavebeenimplicatedinthepathogenesisofrheumatoidarthritis(RA).Theaimofthis studywastosystematicallyanalysethephenotype,cytokineprofileandfrequencyofinterleukin-17(IL-17) producingCD4-positiveTcellsinmononuclearcellsisolatedfromperipheralblood,synovialfluidandsynovial tissueofRApatientswithestablisheddisease,andtocorrelatecellfrequencieswithdiseaseactivity. Methods:Flowcytometrywasusedtoanalysethephenotypeandcytokineproductionofmononuclearcells isolatedfromperipheralblood(PBMC)(n=44),synovialfluid(SFMC)(n=14)andsynovium(SVMC)(n=10)ofRA patientsandPBMCofhealthycontrols(n=13). Results:ThefrequencyofIL-17-producingCD4TcellswaselevatedinRASFMCcomparedwithRAPBMC(P= 0.04).However,thefrequencyofthispopulationinRASVMCwascomparabletothatinpairedRAPBMC.The percentageofIL-17-producingCD4Tcellscoexpressingtumornecrosisfactoralpha(TNFa)wassignificantly increasedinSFMC(P=0.0068).ThefrequencyofIFNg-producingCD4TcellswasalsosignificantlyhigherinSFMC thanpairedPBMC(P=0.042).ThemajorityofIL-17-producingCD4TcellscoexpressedIFNg.IL-17-producingCD4T cellsinRAPBMCandSFMCexhibitedverylittleIL-22orIL-23Rcoexpression. Conclusions:ThesefindingsdemonstrateamodestenrichmentofIL-17-producingCD4TcellsinRASFMC comparedtoPBMC.Th17cellsinSFMCproducemoreTNFathantheirPBMCcounterparts,butarenota significantsourceofIL-22anddonotexpressIL-23R.However,thepercentageofCD4TcellswhichproduceIL-17 intherheumatoidjointislow,suggestingthatothercellsmaybealternativesourcesofIL-17withinthejointsof RApatients. Introduction macrophagesandfibroblasts. Inestablished disease,the