Rhinacanthus nasutus Protects Cultured Neuronal Cells against Hypoxia Induced Cell Death 英文参考文献.docVIP

Molecules 2011, 16, 6322-6338; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Rhinacanthus nasutus Protects Cultured Neuronal Cells against Hypoxia Induced Cell Death James M. Brimson and Tewin Tencomnao * Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, 154 Rama I Rd, Pathumwan, Bangkok 10330, Thailand; E-Mail: James.B@chula.ac.th (J.M.B.) * Author to whom correspondence should be addressed; E-Mail: Tewin.T@chula.ac.th; Tel.: +66-2218-1081 ext. 313; Fax: +66-2-218-1082. Received: 20 July 2011 / Accepted: 21 July 2011 / Published: 26 July 2011 Abstract: Rhinacanthus nasutus (L.) Kurz (Acanthaceae) is an herb native to Thailand and Southeast Asia, known for its antioxidant properties. Hypoxia leads to an increase in reactive oxygen species in cells and is a leading cause of neuronal damage. Cell death caused by hypoxia has been linked with a number of neurodegenerative diseases including some forms of dementia and stroke, as well as the build up of reactive oxygen species which can lead to diseases such as Huntington’s disease, Parkinson’s disease and Alzeheimer’s disease. In this study we used an airtight culture container and the Mitsubishi Gas Company anaeropack along with the MTT assay, LDH assay and the trypan blue exlusion assay to show that 1 and 10 μg mL?1 root extract of R. nasutus is able to significantly prevent the death of HT-22 cells subjected to hypoxic conditions, and 0.1 to 10 μg mL?1 had no toxic effect on HT-22 under normal conditions, whereas 100 μg mL?1 reduced HT-22 cell proliferation. We also used H2DCFDA staining to show R. nasutus can reduce reactive oxygen species production in HT-22 cells. Keywords: Rhinacanthus nasutas; hypoxia; anaeropack; HT-22; antioxidant 1. Introduction Brain ischemia/reperfusion injury is a leading cause in neuronal cell death [1].