Rhn1, a Nuclear Protein, Is Required for Suppression of Meiotic mRNAs in Mitotically Dividing Fission Yeast 英文参考文献.docVIP

Rhn1,aNuclearProtein,IsRequiredforSuppressionof MeioticmRNAsinMitoticallyDividingFissionYeast TomoyasuSugiyama1,2,3*.,RieSugioka-Sugiyama1,2.,KazumasaHada1,2,RyusukeNiwa1,2 1Graduate School ofLifeand EnvironmentalSciences,University ofTsukuba, Tsukuba, Ibaraki,Japan, 2Initiative for thePromotion ofYoung Scientists’Independent Research,UniversityofTsukuba,Tsukuba,Ibaraki,Japan,3PrecursoryResearchforEmbryonicScienceandTechnology(PRESTO),JapanScienceandTechnologyAgency (JST),Kawaguchi,Saitama,Japan Abstract In the fission yeast Schizosaccharomyces pombe, many meiotic mRNAs are transcribed during mitosis and meiosis and selectivelyeliminatedinmitoticcells.However,thispathwayformRNAdecay,calledthedeterminantofselectiveremoval (DSR)-Mmi1 system, targets only some of the numerous meiotic mRNAs that are transcribed in mitotic cells. Here we describeRhn1,anuclearproteininvolvedinmeioticmRNAsuppressioninvegetativefissionyeast.Rhn1ishomologousto buddingyeastRtt103andlocalizestooneorafewdiscretenucleardotsingrowingvegetativecells.Rhn1colocalizeswitha pre-mRNA 39-end processing factor, Pcf11, and with the 59–39 exoribonuclease, Dhp1; moreover, Rhn1 coimmunopreci- pitates with Pcf11. Loss of rhn1 results in elevated sensitivity to high temperature, to thiabendazole (TBZ), and to UV. Interestingly,meioticmRNAs—includingmoa1+,mcp5 ,andmug96+—accumulateinmitoticrhn1Dcells.Accumulationof meioticmRNAsalsooccursinstrainslackingLsk1,akinasethatphosphorylatesserine2(Ser-2)intheC-terminaldomain (CTD)ofRNApolymeraseII(PolII),andinstrainslackingSen1,anATP-dependent59–39RNA/DNAhelicase:notably,both Lsk1andSen1havebeenimplicatedinterminationofPolII-dependenttranscription.Furthermore,RNAiknockdownofcids- + + 2, a Caenorhabditis elegans ortholog of rhn1 , leads to elevated expression of agermline-specific gene, pgl-1, in somatic cells.TheseresultsindicatethatRhn1contributestothesuppressionofmeioticmRNAsinvegetativefissionyeastandthat the mechanism by which Rhn1 downregulates germline-speci