Rice Hypersensitive Induced Reaction Protein 1 (OsHIR1) associates with plasma membrane and triggers hypersensitive cell death 英文参考文献.docVIP

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Rice Hypersensitive Induced Reaction Protein 1 (OsHIR1) associates with plasma membrane and triggers hypersensitive cell death 英文参考文献.doc

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Rice Hypersensitive Induced Reaction Protein 1 (OsHIR1) associates with plasma membrane and triggers hypersensitive cell death 英文参考文献

Zhouetal.BMCPlantBiology2010,10:290 /1471-2229/10/290 RESEARCH ARTICLE OpenAccess RiceHypersensitiveInducedReactionProtein1 (OsHIR1)associateswithplasmamembraneand triggershypersensitivecelldeath LiangZhou1,Ming-YanCheung1,Man-WahLi1,YapingFu2,ZongxiuSun2,Sai-MingSun1,Hon-MingLam1* Abstract Background:Inplants,HIR(HypersensitiveInducedReaction)proteins,membersofthePID(Proliferation,Ionand Death)superfamily,havebeenshowntoplayapartinthedevelopmentofspontaneoushypersensitiveresponse lesionsinleaves,inreactiontopathogenattacks.ThelevelsofHIRproteinswereshowntocorrelatewithlocalized hostcelldeathsanddefenseresponsesinmaizeandbarley.However,notmuchwasknownabouttheHIR proteinsinrice.Sincericeisanimportantcerealcropconsumedbymorethan50%ofthepopulationsinAsiaand Africa,itiscrucialtounderstandthemechanismsofdiseaseresponsesinthisplant.Wepreviouslyidentifiedthe riceHIR1(OsHIR1)asaninteractingpartneroftheOsLRR1(riceLeucine-RichRepeatprotein1).Hereweshowthat OsHIR1triggershypersensitivecelldeathanditslocalizationtotheplasmamembraneisenhancedbyOsLRR1. Result:Throughelectronmicroscopystudiesusingwildtypericeplants,OsHIR1wasfoundtomainlylocalizeto theplasmamembrane,withaminorportionlocalizedtothetonoplast.Moreover,theplasmamembrane localizationofOsHIR1wasenhancedintransgenicriceplantsoverexpressingitsinteractingproteinpartner, OsLRR1.Co-localizationofOsHIR1andOsLRR1totheplasmamembranewasconfirmedbydouble-labeling electronmicroscopy.PathogeninoculationstudiesusingtransgenicArabidopsisthalianaexpressingeitherOsHIR1 orOsLRR1showedthatbothtransgeniclinesexhibitedincreasedresistancetowardthebacterialpathogen Pseudomonassyringaepv.tomatoDC3000.However,OsHIR1transgenicplantsproducedmoreextensive spontaneoushypersensitiveresponselesionsandcontainedlowertitersoftheinvadingpathogen,whencompared toOsLRR1transgenicplants. Conclusion:TheOsHIR1proteinismainlylocalizedtotheplasmamembrane,anditssubcellularlocalizationinthat compartmentisenhancedbyOsLRR1.TheexpressionofOsHIR1maysensitizetheplan