Rising in the East 英文参考文献.docVIP

Petsko Genome Biology 2010,11:102 /2010/11/1/102 COMMENT Rising in the East Gregory A Petsko* e publication of the complete genome sequence of the ordered into larger semi-continuous stretches, called giant panda, Ailuropoda melanoleuca, is a watershed sca? olds, using a variety of bioinformatics tools. A moment for genomics, and not just because of the sca? old will contain a number of contigs separated by technology used. Before I explain, let me say a few words about that technology, because it is worth commenting e sequence, which was published in the 21 January gaps. Larger gaps separate the sca? olds from each other. A good draft sequence of a mammalian genome will have perhaps a hundred sca? olds, or even fewer. Some people issue of Nature (Li et al., Nature 2010 463:311-317, with have wondered whether NGS machines, which typically a nice News and Views piece by Kim Worley and Richard Gibbs on pages 303-304; see also the minireview by Shaun Jackman and Inan? Birol in Genome Biology read less than 100 bases at a time, would ever give comparable accuracy. e Chinese team has answered that question, with a [/2010/11/1/202]), was deter- loud a? rmative. e giant panda sequence has 73-fold mined largely at the Beijing Genomics Institute (more on average redundancy and a median contig length of that later), which is not actually in Beijing, but never 40 kilobases ose are not typographical errors e mind. It’s an important genome, in part because the giant panda is a highly endangered species (only a few thousand are known to exist), in part because on the tree of life the panda sits between the human and the dog, but also because it is the ? rst reported mammalian genome sequence to be determined using so-called ‘next- generation’ sequencing methods.