RNA Aptamers Generated against Oligomeric Aβ40 Recognize Common Amyloid Aptatopes with Low Specificity but High Sensitivity 英文参考文献.docVIP

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RNA Aptamers Generated against Oligomeric Aβ40 Recognize Common Amyloid Aptatopes with Low Specificity but High Sensitivity 英文参考文献.doc

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RNA Aptamers Generated against Oligomeric Aβ40 Recognize Common Amyloid Aptatopes with Low Specificity but High Sensitivity 英文参考文献

RNAAptamersGeneratedagainstOligomericAb40 RecognizeCommonAmyloidAptatopeswithLow SpecificitybutHighSensitivity FaridRahimi1,KazumaMurakami1¤,JamieL.Summers1,Chi-HongB.Chen2,4,GalBitan1,3,4 * 1DepartmentofNeurology,DavidGeffenSchoolofMedicine,UniversityofCaliforniaLosAngeles,LosAngeles,California,UnitedStatesofAmerica, 2Departmentof BiologicalChemistry,UniversityofCaliforniaLosAngeles,LosAngeles,California,UnitedStatesofAmerica,3BrainResearchInstitute,UniversityofCaliforniaLosAngeles, LosAngeles,California,UnitedStatesofAmerica,4MolecularBiologyInstitute,UniversityofCaliforniaLosAngeles,LosAngeles,California,UnitedStatesofAmerica Abstract Aptamersareusefulmolecularrecognitiontoolsinresearch,diagnostics,andtherapy.Despitepromisingresultsinother fields, aptamer use has remained scarce in amyloid research, including Alzheimer’s disease (AD). AD is a progressive neurodegenerativediseasebelievedtobecausedbyneurotoxicamyloidb-protein(Ab)oligomers.Aboligomerstherefore areanattractivetargetfordevelopmentofdiagnosticandtherapeuticreagents.Weusedcovalently-stabilizedoligomersof the40-residueformofAb(Ab40)foraptamerselection.Despitegraduallyincreasingthestringencyofselectionconditions, the selected aptamers did not recognize Ab40 oligomers but reacted with fibrils of Ab40, Ab42, and several other amyloidogenic proteins. Aptamer reactivity with amyloid fibrils showed some degree of protein-sequence dependency. Significantfibrilbindingalsowasfoundforthena?¨velibraryandcouldnotbeeliminatedbycounter-selectionusingAb40 fibrils,suggestingthataptamerbindingtoamyloidfibrilswasRNA-sequence-independent.Aptamerbindingdependedon fibrillogenesis andshowed alagphase. Interestingly, aptamers detectedfibril formation with $15-fold higher sensitivity thanthioflavinT(ThT),revealingsubstantialb-sheetandfibrilformationundetectedbyThT.Thedatasuggestthatunder physiologic conditions, aptamers for oligomeric forms of amyloidogenic proteins cannot be selected due to high, non- specificaff