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RNA-Seq Atlas of Glycine max A guide to the soybean transcriptome 英文参考文献
Severinetal.BMCPlantBiology2010,10:160
/1471-2229/10/160
RESEARCH ARTICLE
OpenAccess
RNA-SeqAtlasofGlycinemax:Aguidetothe
soybeantranscriptome
AndrewJSeverin1*,JennaLWoody1,Yung-TsiBolon2,BinduJoseph1,BrianWDiers3,AndrewDFarmer4,
GaryJMuehlbauer6,RexTNelson5,DavidGrant5,JamesESpecht7,MichelleAGraham1,5,StevenBCannon1,5,
GregoryDMay4,CarrollPVance2,6,RandyCShoemaker1,5
Abstract
Background:Nextgenerationsequencingistransformingourunderstandingoftranscriptomes.Itcandetermine
theexpressionleveloftranscriptswithadynamicrangeofoversixordersofmagnitudefrommultipletissues,
developmentalstagesorconditions.Patternsofgeneexpressionprovideinsightintofunctionsofgeneswith
unknownannotation.
Results:TheRNASeq-Atlaspresentedhereprovidesarecordofhigh-resolutiongeneexpressioninasetof
fourteendiversetissues.Hierarchicalclusteringoftranscriptionalprofilesforthesetissuessuggeststhreecladeswith
similarprofiles:aerial,undergroundandseedtissues.Wealsoinvestigatetherelationshipbetweengenestructure
andgeneexpressionandfindacorrelationbetweengenelengthandexpression.Additionally,wefinddramatic
tissue-specificgeneexpressionofboththemosthighly-expressedgenesandthegenesspecifictolegumesin
seeddevelopmentandnoduletissues.Analysisofthegeneexpressionprofilesofover2,000geneswith
preferentialgeneexpressioninseedsuggeststherearemorethan177geneswithfunctionalrolesthatare
involvedintheeconomicallyimportantseedfillingprocess.Finally,theSeq-atlasalsoprovidesameansof
evaluatingexistinggenemodelannotationsfortheGlycinemaxgenome.
Conclusions:ThisRNA-Seqatlasextendstheanalysesofpreviousgeneexpressionatlasesperformedusing
AffymetrixGeneChiptechnologyandprovidesanexampleofnewmethodstoaccommodatetheincreasein
transcriptomedataobtainedfromnextgenerationsequencing.DatacontainedwithinthisRNA-SeqatlasofGlycine
maxcanbeexploredat/soyseq.
Background
Withthereleaseofahigh-qualitydraftoftheG.max
Early hybridization-based studies indicated that the genomic sequence [6], we are in a position to signifi-
soybean genome
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