RNase 7 Contributes to the Cutaneous Defense against Enterococcus faecium 英文参考文献.docVIP

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RNase 7 Contributes to the Cutaneous Defense against Enterococcus faecium 英文参考文献.doc

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RNase 7 Contributes to the Cutaneous Defense against Enterococcus faecium 英文参考文献

RNase7ContributestotheCutaneousDefenseagainst Enterococcusfaecium BenteKo¨ten1.,MarenSimanski1.,RegineGla¨ser1,RainerPodschun2,Jens-MichaelSchro¨der1,Ju¨rgen Harder1* 1Department of Dermatology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany, 2Institute for Infection Medicine, University Hospital Schleswig- Holstein,CampusKiel,Kiel,Germany Abstract Background:Humanskinisabletomountafastresponseagainstinvadingmicroorganismsbythereleaseofantimicrobial proteinssuchastheribonucleaseRNase7.BecauseRNase7exhibitshighactivityagainstEnterococcusfaeciumtheaimof thisstudywastofurtherexploretheroleofRNase7inthecutaneousinnatedefensesystemagainstE.faecium. Methodology/Principal Findings: Absolute quantification using real-time PCR and ELISA revealed that primary keratinocytesexpressedhighlevelsofRNase7.ImmunohistochemistryshowedRNase7expressioninallepidermallayers of the skin with an intensification in the upper more differentiated layers. Furthermore, RNase 7 was secreted by keratinocytesinvitroandinvivoinasite-dependentway.RNase7wasstillactiveagainstE.faeciumatlowpH(5.5)orhigh NaCl(150mM)concentrationandthebactericidalactivityofRNase7againstE.faeciumrequirednoribonucleaseactivityas shown by recombinant RNase 7 lacking enzymatic activity. To further explore the role of RNase 7 in cutaneous defense againstE.faecium,weinvestigatedwhetherRNase7contributestotheE.faeciumkillingactivityofskinextractsderived fromstratumcorneum.TreatmentoftheskinextractwithanRNase7specificantibody,whichneutralizestheantimicrobial activityofRNase7,diminisheditsE.faeciumkillingactivity. Conclusions/Significance:OurdataindicatethatRNase7contributestotheE.faecium-killingactivityofskinextractsand suggestanimportantroleforRNase7intheprotectionofhumanskinagainstE.faeciumcolonization. Citation:Ko¨tenB,SimanskiM,Gla¨serR,PodschunR,Schro¨derJ-M,etal.(2009)RNase7ContributestotheCutaneousDefenseagainstEnterococcusfaecium .PLoS ONE4(7):e6424.doi:10.1371/journal.pone.0006424 Editor:AdamJ.Ratn