乳腺癌转移相关分子标志物的筛选.pdfVIP

广东药学院学报 }oumal 0/ Gua咱dong Phαrmaceutical University Oct.2015 ,31(5) 乳腺癌转移相关分子标志物的筛选 周澈，吴韵瑶，钟兆健 (广东药学院药科学院，广东广州 51∞06) 摘要:目的筛选与乳腺癌转移相关的分子标志物，为乳腺癌的早期诊断、预后评估以及药物靶点的研究 提供参考。方法从NCBI 的 GEO 数据库中收集乳腺癌相关表达数据，将未转移与已转移的乳腺癌数据 进行对比，采用GeneSpring 软件筛选差异表达基因，并通过 ROC 曲线与主成分分析对得到的差异表达 基因进行评价。结果筛选得到40 个差异表达基因，对乳腺癌转移与未转移样本分类良好。结论筛选 得到的差异基因可作为乳腺癌转移的分子标志物，对乳腺癌是否转移进行预测。 关键词:乳腺癌;转移;差异表达基因;分子标志物 中图分类号:R737.9 文献标志码:A doi: 1O.3969/j.issn.l∞6-8783.2015.05.026 文章编号:1∞6-8783(2015)05-0676-03 Screening of the molecular markers associated with breast cancer metastasis ZHOU Xuan ,WU Yunyao ,ZHONG Zhaojian (School 0/ Pharmacy , Guangdong Phamωceωical University ,Gua昭Zhou 510∞6 ， China) Abstract: Objec创ve To screen the molecular marker百 associated with breast cancer metastasis ,and provide a reference for early diagnosis ,prognosis evaluation and drug target research of breast cancer. Methods The gene expression data of breast cancer was collected from the GEO database of NCBI. 节le non-metastasis and metastasis samples were compared to screen the differential expression genes by Genespring software , and the differential expression genes were evaluated by ROC curves and PCA. Results The screened 40 differential expression genes well classified the non-metastasis and metastasis samples. Conclusion The screened differential expression genes as the molecular markers associated with breast cancer metastasis provide a prediction tool of breast cancer metastasis. Key words: breast cancer; metastasis; differential expression gene; molecular maker 乳腺癌是女性最常见的恶性肿瘤之一，发病率和 基因表达情况。在乳腺癌发生，发展和转移的各个 死亡率居妇女各类恶性肿瘤之首，严重威胁着女性的 阶段，基因表达谱均会发生相应的变化。乳腺癌转 身体健康[1] 。近年来乳腺癌的治疗手段和诊断方法 移早，发展快，甚至在乳腺癌发展初期就已出现微小 转移灶，因此，如能筛选出与乳腺癌