CRISPR Typing and Subtyping for Improved Laboratory Surveillance of Salmonella Infections 英文参考文献.docVIP

CRISPRTypingandSubtypingforImprovedLaboratory SurveillanceofSalmonellaInfections Lae¨titiaFabre1,JianZhang2,GhislaineGuigon3,SimonLeHello1,Ve′roniqueGuibert1,MarieAccou- Demartin1,Sa?¨anadeRomans1,CatherineLim1,ChrystelleRoux1,VirginiePasset3,LaureDiancourt3, MartineGuibourdenche1,SylvieIssenhuth-Jeanjean1,MarkAchtman4,SylvainBrisse3,ChristopheSola2, Franc?ois-XavierWeill1* 1InstitutPasteur,Unite′ desBacte′ries Pathoge`nesEnte′riques,Paris,France,2InstituteofGeneticsandMicrobiology,UMR8621,IGEPETeam,Universud,CNRSUniversite′ Paris-Sud11,Orsay,France,3InstitutPasteur,GenotypingofPathogensandPublicHealth,Paris,France,4EnvironmentalResearchInstitute,UniversityCollegeCork,Cork, Ireland Abstract Laboratorysurveillancesystemsforsalmonellosisshouldideallybebasedontherapidserotypingandsubtypingofisolates. However,currenttypingmethodsarelimitedinbothspeedandprecision.Using783strainsandisolatesbelongingto130 serotypes,weshowherethatanewfamilyofDNArepeatsnamedCRISPR(clusteredregularlyinterspacedshortpalindromic repeats) is highly polymorphic in Salmonella. We found that CRISPR polymorphism was strongly correlated with both serotype and multilocus sequence type. Furthermore, spacer microevolution discriminated between subtypes within prevalentserotypes,makingitpossibletocarryouttypingandsubtypinginasinglestep.Wedevelopedahigh-throughput subtypingassayforthemostprevalentserotype,Typhimurium.Anopenweb-accessibledatabasewassetup,providinga serotype/spacer dictionary and an international tool for strain tracking based on this innovative, powerful typing and subtypingtool. Citation: Fabre L, Zhang J, Guigon G, Le Hello S, Guibert V, et al. (2012) CRISPR Typing and Subtyping for Improved Laboratory Surveillance of Salmonella Infections.PLoSONE7(5):e36995.doi:10.1371/journal.pone.0036995 Editor:IgorMokrousov,St.PetersburgPasteurInstitute,RussianFederation ReceivedFebruary23,2012;AcceptedApril11,2012;PublishedMay18,2012 Copyright: ? 2012 Fabre et al. This is an open-access article