Room-temperature super-extraction system(RTSES) optimizes the anxiolytic- andantidepressant-like behavioural effects of traditionalXiao-Yao-San in mice 英文参考文献.docVIP

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Room-temperature super-extraction system(RTSES) optimizes the anxiolytic- andantidepressant-like behavioural effects of traditionalXiao-Yao-San in mice 英文参考文献.doc

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Room-temperature super-extraction system(RTSES) optimizes the anxiolytic- andantidepressant-like behavioural effects of traditionalXiao-Yao-San in mice 英文参考文献

Yinetal.ChineseMedicine2012,7:24 /content/7/1/24 RESEARCH OpenAccess Room-temperaturesuper-extractionsystem (RTSES)optimizestheanxiolytic-and antidepressant-likebehaviouraleffectsof traditionalXiao-Yao-Saninmice Shih-HsiYin1,Ching-ChengWang1,Tain-JunnCheng2,3,4,5,Chia-YuChang2,6,7,Kao-ChangLin2,7,Wei-ChihKan8,9, Hsien-YiWang8,9,WennyMei-WenKao10,11,Yen-LiangKuo11,Jian-ChyiChen7,Shun-LaiLi7,Chia-HuiCheng7 andJiunn-JyeChuu7,12* Abstract Background:Xiao-Yao-San(XYS)isaChinesemedicinalformulafortreatinganxietyanddepression.Thisstudyaims toevaluatetheuseofaroom-temperaturesuper-extractionsystem(RTSES)toextractthemajoractivecomponents ofXYSandenhancetheirpsycho-pharmacologicaleffects. Methods:TheneuroprotectiverolesofXYS/RTSESagainstreserpine-derivedneurotoxicitywereevaluatedusinga glialcellinjurysystem(invitro)andadepression-likeC57BL/6Jmousemodel(invivo).Theanxiolytic-behavioural effectsweremeasuredbytheelevatedplus-maze(EPM)testandtheantidepressanteffectswereevaluatedbythe forcedswimmingtest(FST)andtailsuspensiontest(TST).Glucosetoleranceandinsulinresistancewereassayedby ELISA.Theexpressionof5-HT1Areceptorsintheprefrontalcortexwasexaminedbywesternblotting. Results:XYS/RTSES(300μg/mL)diminishedreserpine-inducedglialcelldeathmoreeffectivelythaneitherXYS (300μg/mL)orfluoxetine(30μM)at24h(P=0.0481andP=0.054,respectively).OraladministrationofXYS/RTSES (500mg/kg/day)for4consecutiveweekssignificantlyelevatedtheratiosofentries(openarms/closedarms; P=0.0177)andshuttleactivity(P=0.00149)ontheEPMtest,andreducedtheimmobilitytimeby90%ontheTST (P=0andFST(P=0.0000053839).XYS/RTSESalsoimprovedtheregulationofbloodglucose(P=0.0305) andincreasedtheinsulinsensitivity(P=0.0093).TheWesternblotresultsindicatedthattheactivationofcerebral 5-HT1AreceptorsmaybeinvolvedinthemechanismsofXYS/RTSESactions. Conclusion:TheRTSEScouldprovideanovelmethodforextractingeffectiveanxiolytic-andantidepressant-like substances.XYS/RTSESimprovedtheregulationofbloodglucoseandincreasedtheinsulinsensitiv