Running Worms C. elegans Self-Sorting by Electrotaxis 英文参考文献.docVIP

RunningWorms:C.elegansSelf-SortingbyElectrotaxis XavierManie`re1,Fe′lixLebois2,IvanMatic1,BenoitLadoux2,Jean-MarcDiMeglio2,PascalHersen2* 1LaboratoireTaMaRa,U1001Universite′ ParisDescartesandINSERM,Paris,France,2LaboratoireMatie`re etSyste`mes Complexes,UMR7057Universite′ ParisDiderotand CNRS,Ba?timent Condorcet,Paris,France Abstract ThenematodeC.elegansdisplayscomplexdynamicalbehaviorsthatarecommonlyusedtoidentifyrelevantphenotypes. Althoughitsmaintenanceisstraightforward,sortinglargepopulationsofwormswhenlookingforabehavioralphenotype is difficult, time consuming and hardly quantitative when done manually. Interestingly, when submitted to a moderate electricfield,wormsmovesteadilyalongstraighttrajectories.Here,wereportaninexpensivemethodtomeasureworms crawlingvelocitiesandsortthemwithinafewminutesbytakingadvantageoftheirelectrotacticskills.Thismethodallows toquantitativelymeasuretheeffectofmutationsandagingonworm’scrawlingvelocity.Wealsoshowthatwormswith differentlocomotoryphenotypescanbespatiallysorted,fastwormstravelingawayfromslowones.Groupofnematodes with comparable locomotory fitness could then be isolated for further analysis. C. elegans is a growing model for neurodegenerativediseasesandusingelectrotaxisforself-sortingcanimprovethehigh-throughputsearchoftherapeutic bio-molecules. Citation: Manie`re X, Lebois F, Matic I, Ladoux B, Di Meglio J-M, et al. (2011) Running Worms: C. elegans Self-Sorting by Electrotaxis. PLoS ONE 6(2): e16637. doi:10.1371/journal.pone.0016637 Editor:PankajKapahi,BuckInstituteforAgeResearch,UnitedStatesofAmerica ReceivedSeptember20,2010;AcceptedJanuary6,2011;PublishedFebruary4,2011 Copyright: ? 2011 Manie`re et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: Some nematode strains used in this work were provided by the Caenorhabditis Genetics