S Phase–Coupled E2f1 Destruction Ensures Homeostasis in Proliferating Tissues 英文参考文献.docVIP

下载本文档

5
0
约7.29万字
约 17页
2017-05-13 发布于上海
举报
版权申诉

S Phase–Coupled E2f1 Destruction Ensures Homeostasis in Proliferating Tissues 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

S Phase–Coupled E2f1 Destruction Ensures Homeostasis in Proliferating Tissues 英文参考文献

SPhase–CoupledE2f1DestructionEnsuresHomeostasis inProliferatingTissues JeanM.Davidson1,RobertJ.Duronio1,2,3* 1DepartmentofBiology,TheUniversityofNorthCarolinaatChapelHill,ChapelHill,NorthCarolina,UnitedStatesofAmerica,2LinebergerComprehensiveCancerCenter, TheUniversityofNorthCarolinaatChapelHill,ChapelHill,NorthCarolina,UnitedStatesofAmerica,3PrograminMolecularBiologyandBiotechnology,TheUniversityof NorthCarolinaatChapelHill,ChapelHill,NorthCarolina,UnitedStatesofAmerica Abstract Precisecontrolofcellcycleregulatorsiscriticalfornormaldevelopmentandtissuehomeostasis.E2Ftranscriptionfactors areactivatedduringG1todrivetheG1-StransitionandaretheninhibitedduringSphasebyavarietyofmechanisms.Here, wegeneticallymanipulatethesingleDrosophilaactivatorE2F(E2f1)toexplorethedevelopmentalrequirementforSphase– coupled E2F down-regulation. Expression of an E2f1 mutant that is not destroyed during S phase drives cell cycle progression and causes apoptosis. Interestingly, this apoptosis is not exclusively the result of inappropriate cell cycle progression,becauseastableE2f1mutantthatcannotfunctionasatranscriptionfactorordrivecellcycleprogressionalso triggersapoptosis.ThisobservationsuggeststhattheinappropriatepresenceofE2f1proteinduringSphasecantrigger apoptosisbymechanismsthatareindependentofE2Factingdirectlyattargetgenes.TheabilityofSphase-stabilizedE2f1 totriggerapoptosisrequiresaninteractionbetweenE2f1andtheDrosophilapRbhomolog,Rbf1,andinvolvesinductionof the pro-apoptotic gene, hid. Simultaneously blocking E2f1 destruction during S phase and inhibiting the induction of apoptosisresultsintissueovergrowthandlethality.WeproposethatinappropriateaccumulationofE2f1proteinduringS phase triggers the elimination of potentially hyperplastic cells via apoptosis in order to ensure normal development of rapidlyproliferatingtissues. Citation: Davidson JM, Duronio RJ (2012) S Phase–Coupled E2f1 Destruction Ensures Homeostasis in Proliferating Tissues. PLoS Genet 8(8): e1002831. doi:10.1371/jo