SAGE profiling of the forelimb and hindlimb 英文参考文献.docVIP

/2002/3/3/reviews/1007.1 Minireview SAGE profiling of the forelimb and hindlimb Malcolm Logan Address: Division of Developmental Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK. E-mail: mlogan@nimr.mrc.ac.uk Published: 27 February 2002 GenomeBiology 2002, 3(3):reviews1007.1–1007.3 The electronic version of this article is the complete one and can be found online at /2002/3/3/reviews/1007 ? BioMed Central Ltd (Print ISSN 1465-6906; Online ISSN 1465-6914) Abstract A recent study has used serial analysis of gene expression to compare mouse forelimb and hindlimb gene-expression profiles. The method successfully identified known regulators of limb identity and has generated a candidate set of differentially expressed genes that may regulate limb identity. Patterning of tissues during embryonic development between them. SAGE involves the production of short sequence tags of around 10 base-pairs (bp) from cDNAs derived from the tissue of interest. Each tag contains suffi- cient information to identify it as representing a particular transcript. Many transcript tags are concatenated and cloned to generate a SAGE library. Library clones are then sequenced to reveal the identity of multiple tags simultane- ously [8]. The presence and abundance of tags representing a particular gene in a SAGE library provides both a qualita- tive and a quantitative readout of gene expression in the original source. Moreover, the comparison of tags from one library to another can, in the most comprehensive examples, provide accurate gene-expression profiles and enable the genetic regulators of cellular difference to be determined. requires the coordination of cell-fate specification with rates of cell proliferation and apoptosis. The developing vertebrate limb is a favored model system for studying the fundamental processes involved in normal embryo development.