Salidroside Attenuates Hydrogen Peroxide-Induced Cell Damage Through a cAMP-Dependent Pathway 英文参考文献.docVIP

Molecules 2011, 16, 3371-3379; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Salidroside Attenuates Hydrogen Peroxide-Induced Cell Damage Through a cAMP-Dependent Pathway Shuang Guan 1, Wei Wang 1,?, Jing Lu 1,2,?, Wenhui Qian 2, Guoren Huang 2, Xuming Deng 1,* and Xuelin Wang 1,* 1 Institute of Zoonoses, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China; E-Mails: gshuang1973@126.com. (S.G.); wangwei@ (W.W.) 2 Laboratory of Nutrition and Function Food, Jilin University, Changchun, Jilin 130062, China; E-Mail: xlujing1@ (J.L.) ? These authors contributed equally to this work. * Authors to whom correspondence should be addressed; E-Mails: xumingdeng@ (X.D.); wxlwqx@ (X.W.); Tel.: +86-431-8783-6161; Fax: +86-431-8783-6160. Received: 25 March 2011; in revised form: 8 April 2011 / Accepted: 8 April 2011 / Published: 19 April 2011 Abstract: Salidroside, a major component of Rhodiola rosea L., has shown various pharmacological functions, including antioxidant effects, but the signal transduction pathway of its antioxidant effects is not very clear. In this study, we found that salidroside could attenuate hydrogen peroxide (H2O2)-induced HL-7702 cell damage, inhibit H2O2-induced cytosolic free Ca2+ ([Ca2+]i) elevation, scavenge reactive oxygen species (ROS) and increase 3’-5’-cyclic adenosine monophosphate (cAMP) level in a dose-dependent manner, but it couldn’t influence 3’-5’-cyclic guanosine monophosphate (cGMP) levels. Therefore, these results indicated that the antioxidant effects of salidroside were associated with down-regulation of [Ca2+]i, ROS occur via a cAMP-dependent pathway. Keywords: salidroside; antioxidant; AMP; cGMP; [Ca2+]i; ROS  Molecules 2011, 16 3372 1. Introduction Rhodiola rosea L. has been widely used for a long time in Traditional Chinese Medicine. Salidroside (р-hydr