Salivary Aldehyde Dehydrogenase Activity towards Aromatic Aldehydes and Comparison with Recombinant ALDH3A1 英文参考文献.docVIP

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Salivary Aldehyde Dehydrogenase Activity towards Aromatic Aldehydes and Comparison with Recombinant ALDH3A1 英文参考文献.doc

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Salivary Aldehyde Dehydrogenase Activity towards Aromatic Aldehydes and Comparison with Recombinant ALDH3A1 英文参考文献

Molecules 2009, 14, 2363-2372; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Salivary Aldehyde Dehydrogenase: Activity towards Aromatic Aldehydes and Comparison with Recombinant ALDH3A1 Joanna Giebu?towicz 1, Renata Wolinowska 2, Anna Sztybor 3, Monika Pietrzak 3, Piotr Wroczyński 1 and Jacek Wierzchowski 3,* 1 Department of Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha Street, PL-02-097, Warsaw, Poland; E-mail: joanna.giebultowicz@ (J.G.) 2 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Warsaw, 3 Oczki Street, PL-02-007, Warsaw, Poland; E-mail: rwolinowska@.pl (R.W.) 3 Department of Biophysics, University of Warmia and Mazury, 4 Oczapowskiego St., PL-10-719 Olsztyn, Poland * Author to whom correspondence should be addressed; E-mail: jacek.wie@.pl; Tel. +48-89-523-3324; Fax: +48-89-523-3408 Received: 11 May 2009; in revised form: 15 June 2009 / Accepted: 18 June 2009 / Published: 2 July 2009 Abstract: A series of aromatic aldehydes was examined as substrates for salivary aldehyde dehydrogenase (sALDH) and the recombinant ALDH3A1. Para-substituted benzaldehydes, cinnamic aldehyde and 2-naphthaldehydes were found to be excellent substrates, and kinetic parameters for both salivary and recombinant ALDH were nearly identical. It was demonstrated that for the fluorogenic naphthaldehydes the only produced reaction product after incubation in saliva is the carboxylate. Keywords: saliva; aldehyde dehydrogenase; enzyme kinetics; aromatic aldehydes 1. Introduction Human saliva exhibits significant activity of many detoxifying enzymes, including aldehyde dehydrogenase (ALDH, E.C., cf. Scheme 1), which presumably protects the organism from various aldehydes contained in food either as natural ingredients or as contaminants [1-4]. Salivary aldehyde dehydrogenase probably consists of one isozyme, classified